林业科学2017，Vol.53Issue(9)：35-44,10.DOI:10.11707/j.1001-7488.20170905
转桑树MAPK5基因拟南芥在不同逆境胁迫下的抗性分析
Resistance of Arabidopsis thaliana Transformed with Mulberry MAPK5 under Stress Conditions
摘要
Abstract
[Objective] Mitogen-activated protein kinases ( MAPK ) play key roles in response to biotic and abiotic stresses in plants. To investigate the function of mulberry ( Morus notabilis) MnMAPK5 in response to stress conditions, the activities of the promoter of MnMAPK5 in transgenic Arabidopsis thaliana under stress conditions, subcellular localization of MnMAPK5 and its overexpression in A. thaliana were analyzed.[Method] The promoter of MnMAPK5 was inserted into the plant expression vector pBI121 and transformed into A. thaliana by the floral dip method . The activity of GUS was dectected in the transgenic plants treated respectively with high temperature,low temperature,NaCl and PEG. The overexpression vector was constructed and transformed into A. thaliana and the T3 transgenic plants were used for resistance analysis.[Result] MnMAPK5 gene was found encoding a cytoplasm-and nucleus-localized protein. The results of GUS staining showed that the activity of MnMAPK5 promoter was induced by high temperature,low temperature,salt and drought. The MnMAPK5-overexpressed transgenic A. thaliana showed lower germination rates,shorter length,and worse growth than wild A. thaliana plants under salt,drought,low temperature and H2 O2 . Under salt stress,MDA content and POD activity were higher in the MnMAPK5-overexpressed transgenic plants while CAT activity,soluble sugar content and H2 O2 content were lower. Under drought,the MDA content and H2 O2 content in the MnMAPK5-overexpressed transgenic plants were higher than in the wild plants while CAT activity and POD activity were lower. Under low temperature stress,CAT activity,POD activity,proline content and soluble sugar content in the transgenic plants were lower than in the wild plants. Under H2 O2 stress,MDA content was higher in the transgenic plants than in the wild plants while proline content and soluble sugar content were lower. Besides,the expression levels of AtPOD,AtCAT,AtRD22 and other stress related genes were lower in the transgenic plants than in the wild plants under all the above stresses.[Conclusion] The activities of MnMAPK5 promoter in the transgenic A. thaliana showed responses to different abiotic stresses. The MnMAPK5-overexpressed transgenic A. thaliana showed declined resistance against abiotic stresses,and indicated that MnMAPK5 may be a negative factor in response to stress conditions.关键词
桑树/MAPK/非生物胁迫/胁迫响应/启动子Key words
Morus/MAPK/abiotic stress/stress responses/promoter分类
农业科技引用本文复制引用
刘长英,张梦,魏从进,许亚珍,曹博宁,郑莎,范伟,向仲怀,赵爱春..转桑树MAPK5基因拟南芥在不同逆境胁迫下的抗性分析[J].林业科学,2017,53(9):35-44,10.基金项目
国家现代农业产业技术体系建设专项(CARS-18-ZJ0201) （CARS-18-ZJ0201）
国家农业部公益性行业(农业) 科研专项(201403064). （农业）
