热带作物学报2017，Vol.38Issue(7)：1235-1242,8.DOI:10.3969/j.issn.1000-2561.2017.07.010
胡椒连作对土壤微生物群落功能多样性与群落结构的影响
Effects of Consecutive Monoculture of Piper nigrum L. on Soil Microbial Functional Diversity and Community Structure
摘要
Abstract
Consecutive monoculture induces black pepper (Piper nigrum L.) poor growth, low yield, and serious soil-borne disease, thus becomes one of the most serious threats to the sustainable development of black pepper industry in China. To investigate its microbiological formation mechanisms, the soil samples from black pepper orchards with consecutive monoculture for 8, 18 and 28 years were analyzed by using the BIOLOG EcoPlates method and the responses of soil microbial functional diversity and community structure to different planting ages were demonstrated as follow. It revealed that average well color development ( AWCD) obviously decreased with increasing planting duration. The same tendency was also demonstrated in soil microbial diversity indices, such as Shannon index, Shannon evenness index and Simpson index. In addition, the utilization of carbon sources by soil microbes from different planting duration showed significantly different. For example, the utilization rates of phenolic compounds and polymers obviously decreased during the period from the 8 th year to the 18th year and those of carbohydrates, amino acids, carboxylic acids and amines obviously decreased from the 18th year to the 28th year, which indicating the soil microbial community structure experienced two obvious changes with planting duration increasing. Furthermore, the principal component analysis ( PCA) also showed the soil samples from the three planting duration were clearly separated from each other. These results suggested that consecutive monoculture of P. nigrum L. could decrease soil microbial activity and diversity, change the soil microbial community.关键词
胡椒/连作障碍/BIOLOG/微生物功能多样性/微生物群落结构Key words
Black pepper (Piper nigrum L.)/obstacle of consecutive monoculture/BIOLOG analysis/soil microbial functional diversity/community structure分类
农业科技引用本文复制引用
王灿,李志刚,杨建峰,祖超,郑维全,邬华松,鱼欢..胡椒连作对土壤微生物群落功能多样性与群落结构的影响[J].热带作物学报,2017,38(7):1235-1242,8.基金项目
国家自然科学基金(No. 31601837) （No. 31601837）
中央级公益性科研院所基本科研业务费专项资金(No. 1630142017012). （No. 1630142017012）
