热带作物学报2017，Vol.38Issue(7)：1285-1294,10.DOI:10.3969/j.issn.1000-2561.2017.07.017
基于转录组的芒果MYB家族基因的鉴定及分析
Identification and Analysis of MYB Transcription Factor Family Based on Transcriptome Data in Mango (Mangifera indica L.)
摘要
Abstract
As one of the large transcription factor families, MYB family is involved in regulating a variety of physiological processes in plants. Based on the results of transcriptome sequencing of the fruits in mango, 71 sequences of MYB family proteins, including one 4R-MYB protein, three R1R2R3-MYB proteins, 60 R2R3-MYB proteins and 7 MYB related proteins, were identified in the study. The phylogenetic tree and motifs analyses showed that, with a few individual exceptions, MYB proteins of the same type clustered together, and the majority of the close members in the phylogenetic tree exhibited same or similar motifs compositions. 60 full-length MYB proteins were analyzed, in which most were unstable, and all of those proteins were hydrophilic proteins, without a signal-peptide and transmembrane region. Subcellular localization analysis indicated that those proteins were located in nucleus. In addition, the phylogenetic analyses of the mango ( 60 members) and Arabidopsis ( 126 members) R2R3-MYB proteins showed that those proteins were highly conserved between mango and Arabidopsis. Conserved domain ( R2 and R3 repeats) of mango R2R3-MYB proteins contained multiple conserved amino residues. GO (Gene Ontology) annotation showed that those proteins could be categorized into 15 function subgroups of three main categories: biological processes, cellular components and molecular function.关键词
芒果/MYB家族基因/R2R3-MYB/生物信息学Key words
Mango (Mangifera indica L.)/MYB family genes/R2R3-MYB/bioinformatics分类
农业科技引用本文复制引用
郑斌,武红霞,王松标,马小卫,许文天,罗纯,姚全胜..基于转录组的芒果MYB家族基因的鉴定及分析[J].热带作物学报,2017,38(7):1285-1294,10.基金项目
农业行业科研专项子项目(No. 201203092-3) （No. 201203092-3）
广东省自然科学基金-粤西北创新人才专项(No. 2016A030307006). （No. 2016A030307006）
