热带作物学报2017，Vol.38Issue(7)：1295-1302,8.DOI:10.3969/j.issn.1000-2561.2017.07.018
甘蔗花青素转录因子基因ScRS克隆与基因枪转化研究
Cloning of Sugarcane Transcriptional Factor Gene ScRS and Transformation by Microprojectile Bombardment
摘要
Abstract
Anthocyanins are not only natural pigments, but also health care ingredients. The anthocyanin transcriptional factor genes play import roles in anthocyanin biosynthesis. In this study, the specific primer was designed according to the sequence of maize anthocyanin transcriptional factor gene RS. The full sequence of putative sugarcane anthocyanin transcription factor gene ScRS was cloned by homology cloning technology. The plant expression vector of pCAMBIA1301-35SN-ScRS was constructed by adding endonuclease sites of KpnⅠ and SalⅠ at the ends of ScRS sequence, which was transformed into sugarcane calli via gene gun bombardment, and the calli bombarded turned purple-red. The calli with purple-red color were selected and regenerated into plants. The PCR results were positive in the transgenic detection of the regenerated sugarcane plants with specific primers of ScRS. The results preliminarily confirmed that the transformation of ScRS gene would make sugarcane calli colorated. Cloning and characterization of anthocyanin transcription factor genes would contribute to develop anthocyanin-rich varieties and establish visual tracing system in genetic transformation.关键词
甘蔗/花青素转录因子基因/ScRS/基因枪轰击Key words
Sugarcane/anthocyanin transcriptional factor gene/ScRS/microprojectile bombardment分类
农业科技引用本文复制引用
刘迪,许莉萍,张华,施桂姣,肖乃衍,Khalil Farghama,许跃语,陈平华,张卓,王恒波,高三基..甘蔗花青素转录因子基因ScRS克隆与基因枪转化研究[J].热带作物学报,2017,38(7):1295-1302,8.基金项目
福建省自然科学基金(No. 2014J01092) （No. 2014J01092）
国家自然科学基金项目(No. 31070330) （No. 31070330）
科技发展基金项目(No. KF2015080, KF2015118). （No. KF2015080, KF2015118）
