热带作物学报2017，Vol.38Issue(7)：1316-1322,7.DOI:10.3969/j.issn.1000-2561.2017.07.021
利用SSR分子标记研究白菜类亚种资源的遗传多样性
Genetic Diversity Analysis in Chinese Cabbage [ssp. chinensis (L.) Makino] Resources Based on SSR Molecular Markers
摘要
Abstract
In order to analyze the evolution of Chinese cabbage [ssp. chinensis (L.) Makino] resource, a total of 94 Chinese cabbage [ssp. chinensis (L.) Makino] materials were used to assess the genetic diversity. The results showed that, nineteen cabbage SSR primers combinations amplified 137 alleles, 7.16 loci on average for each pair of primers. The average of polymorphism loci percentage ( P), genetic heterozygosity ( He), effective number of alleles (Ne), Shannon-Wiener index (H') and polymorphism information content (PIC) was 99.9%, 0.897, 11.598, 2.454, 0.886, respectively, and there were extremely significant correlation among them except P value. 94 Chinese cabbage [ssp. chinensis (L.) Makino] materials were clustered into three groups by UPGMA when GSC was 0.58, much of local Chinese cabbage was closer relationships with pakchoi. The order of genome amplification polymorphism by Chinese cabbage SSR primers was (small) cabbage, red bolt second lowest and wild mustard.关键词
白菜类亚种资源/SSR/遗传多样性/亲缘关系Key words
Chinese cabbage [ssp. chinensis (L.) Makino] resource/SSR/genetic diversity/genetic relationship分类
农业科技引用本文复制引用
李光光,黄红弟,张华,郑岩松,李荣华..利用SSR分子标记研究白菜类亚种资源的遗传多样性[J].热带作物学报,2017,38(7):1316-1322,7.基金项目
广东省自然科学基金(No. 2015A030313500) （No. 2015A030313500）
广东省普通高校国际暨港澳台合作创新平台及国际合作重大项目(No. 2015KGJHZ015) （No. 2015KGJHZ015）
广东省科技计划项目(No. 2016B020201001) （No. 2016B020201001）
广州市科技计划项目(No. 201604020084) （No. 201604020084）
广州市属高校科技计划项目(No. 1201420621) （No. 1201420621）
广州市支农资金项目. （）
