热带作物学报2017，Vol.38Issue(8)：1383-1389,7.DOI:10.3969/j.issn.1000-2561.2017.08.001
辐射花粉诱导木薯无融合生殖及鉴定研究
Induction and Identification of Apomixis by Radiation Pollen in Cassava
摘要
Abstract
Pollen induction is an important way to obtain apomixis. In this study, the pollen which radiated was used to pollinate with female flowers for inducing cassava apomixis in the living body, and acquired the sporophyte apomixis germplasmthe induced by the pollen that radiated under 60Coγ-ray. The results showed that:The way to induce cassava apomixis by 60Coγ-ray radiation pollen was effective, and the choice of radiation dose and variety had a certain influence on the induction of cassava apomixis. A total of 23 plants were harvested in this study, and the offsprings were detected by cytological and codominant EST-SSR markers, it showed that the 23 offsprings were heterozygous diploid; Furthermore, SRAP molecular markers were used to identify heterozygous heterozygosity or homozygous heterozygosity for each progeny, and two offsprings were found to be genetic homogeneity of SC5.关键词
木薯/辐射花粉/无融合生殖/倍性鉴定/分子标记Key words
Manihot esculenta Crantz/radiated pollen/apomixis/ploidy identification/molecular markers分类
农业科技引用本文复制引用
孙琪,陈霞,贾泽冲,刘云豪,赖杭桂,陈松笔,朱文丽..辐射花粉诱导木薯无融合生殖及鉴定研究[J].热带作物学报,2017,38(8):1383-1389,7.基金项目
国家自然科学基金项目(No. 31360354) （No. 31360354）
中国热带农业科学院基本科研业务费专项资金(No. 1630032017089) （No. 1630032017089）
海南大学科研启动基金项目(No. kyqd1644) （No. kyqd1644）
海南省普通高等学校研究生创新科研课题(No. Hys2017-72). （No. Hys2017-72）
