热带作物学报2017，Vol.38Issue(8)：1390-1394,5.DOI:10.3969/j.issn.1000-2561.2017.08.002
毛酸浆2个栽培品种的核型分析
Karyotype Analysis of Two Cultivars Physali pubescens L.
祁宏英 1徐洪国 1顾灵杰1
作者信息
- 1. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006
- 折叠
摘要
Abstract
In this paper, the experimental materials were the root tips of two cultivars from Physalis pubescens L. The methods of pretreatment, dissociation and chromosome sectioning technique were studied for the karyotype analysis. The root tips of P. pubescens L. were pretreated with 0.1% colchicines and 0.002 mol/L 8-hydroxyquinoline (1:1) for 3 h at 4℃. The root tips were dissociated in 1 N HCl at 60℃ for 3 min, and then the root tips were enzymatic hydrolyzed with mixed enzymes. The results showed the images of the chromosome were clear. The chromosome number of the two cultivars were both 2n=24, and which were both small chromosome. However, the karyotypes were different. P. pubescens 'Tieba': The chromosome length was 30.72μm, and the variation range of the chromosome length was 1.61~3.42μm. The karyotype formula was 2n=2sm+22m, which belongs to "1B" type, as the karyotype asymmetry index was 56.35%. P. pubescens 'Lilitian': The chromosome length was 28.42μm, and the variation range of the chromosome length was 1.52~3.32 μm. The karyotype formula was 2n=2sm+2M+20m, which belongs to "2B" type, as the karyotype asymmetry index was 54.93%.关键词
毛酸浆/染色体/核型分析Key words
Physali pubescens L./chromosome preparation/karyotype analysis分类
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祁宏英,徐洪国,顾灵杰..毛酸浆2个栽培品种的核型分析[J].热带作物学报,2017,38(8):1390-1394,5.
