热带作物学报2017，Vol.38Issue(8)：1512-1518,7.DOI:10.3969/j.issn.1000-2561.2017.08.022
2株毒死蜱降解菌的分离鉴定及其混合降解特性研究
Isolation, Identification of Two Chlorpyrifos-degrading Strains and Their Mixed Biodegradation Characteristics of Chlorpyrifos
摘要
Abstract
Two chlorpyrifos-degrading bacterium strains, D1 and D3, were isolated from the activated sludge of an insecticide factory. Based on the phylogenetic analysis of 16S rRNA gene sequences, strain D1 was identified as Ochrobactrum sp., strain D3 was identified preliminarily as Paracoccus sp.. When the mixed ratio of D1:D3 was 1:1.25, the degradation rate of chlorpyrifos was improved by 12%-26%, significantly superior to that of single strain. The optimal temperature, initial pH and salt concentration of the medium for chlorpyrifos degradation by mixed D1 and D3 were 30℃, pH 7.0 and 0.5% NaCl, respectively. The high colonizing ability and chlorpyrifos-degrading rate of mixed D1 and D3 was maintained after the application of the bacterial solution into soil.关键词
毒死蜱/降解菌株的分离鉴定/混合降解/降解特性Key words
Chlorpyrifos/isolation and identification of degrading-strain/mixed biodegradation/biodegradation characteristics分类
农业科技引用本文复制引用
李怡,武春媛,李玮,李勤奋..2株毒死蜱降解菌的分离鉴定及其混合降解特性研究[J].热带作物学报,2017,38(8):1512-1518,7.基金项目
中央级公益性科研院所基本科研业务费专项(No. 2014hzs1J005) （No. 2014hzs1J005）
公益性行业(农业)科研专项(No. 201503107-14) （农业）
海南省重大科技计划(2017). （2017）
