热带作物学报2017，Vol.38Issue(8)：1519-1528,10.DOI:10.3969/j.issn.1000-2561.2017.08.023
防治木薯细菌性枯萎病的生防菌株筛选和防效测定
Selection and Identification of Biocontrol Agents Against Cassava Blight Bacteria
摘要
Abstract
298 bacterial isolates were recovered from eight different microenvironments of field-grown cassava plants. of that, 160 (53.69%) expressed protease, 57 (19.13%) produced cellulase, 7 (2.35%) expressed chitinase, and 203 (68.12%) secreted siderophores; 11 (3.69%) produced glucanase, 150 (50.34%) expressed IAA, and only 9 (3.02%) representative isolates were for in vitro antagonism. ARDRA and identification of antagonistic bacteria identified 21 distinct groups ( G1-21) with the similarity coefficient of 50% among the 298 isolates, BOX-PCR fingerprint analysis revealed that isolates in different ARDRA groups had dissimilar genotypes. Eleven representative isolates were evaluated for their efficacy against cassava blight bacterial mildew caused by Xanthomonas axonopodis pv. Manihotis in the greenhouse, the isolates showed biocontrol efficacy ranging from 44.14% to 100%. Eight of them (HWY-3-1 (100%), DBS-5 (85.96%), HS-4-3 (82.88%), DHWP-1 (79.37%), HWYT-3-2 (77.88%), HWS-4-3 (77.84%), DHNS-3-5 (65.34%), DHWR-5-1 (61.83%) attained the efficacy over 60%. HWYT-3-2 and HNR-3-7 significantly suppressed the disease at 30 dpt, and HWYT-3-2 provided significant disease protection from 30-40 dpt, the average bio control efficiency was 38.11%. The strategy of the study was feasible, and found one strains HWYT-3-2 Bacillus pumilus of BCAs against CBB control.关键词
木薯细菌性枯萎病/分离和筛选/颉颃/聚类分析/生防效果Key words
Cassava bacterial blight/selection and identification/antagonism activity/cluster analyse/bicontrol efficiency分类
农业科技引用本文复制引用
郑丽,覃新导,张海鹏,陈阳,刘孟浩,宋艳培,江绍锋,林江,高赛超,何时雨..防治木薯细菌性枯萎病的生防菌株筛选和防效测定[J].热带作物学报,2017,38(8):1519-1528,10.基金项目
海南省自然科学基金项目 "芽孢杆菌对木薯细菌性枯萎病防效及叶围微生态影响研究" (No. 20153128) （No. 20153128）
热科院生物所中央级公益性科研院所基本科研业务费专项 "木薯细菌性枯萎病生防菌株筛选及机理研究"(No. ITBB140306) （No. ITBB140306）
现代农业产业技术体系建设专项资金(No. CARS-12). （No. CARS-12）
