热带作物学报2017，Vol.38Issue(8)：1565-1571,7.DOI:10.3969/j.issn.1000-2561.2017.08.030
固相萃取法与QuEChERS法对比检测无核荔枝中19种农药残留
Determination of 19 Pesticides Residues in Seedless-Litchi by Gas Chromatography-Tandem Mass Spectrometry
摘要
Abstract
Two methods of sample preparation were developed for simultaneous analysis of 19 pesticides residues in seedless-litchi in the study. Factors affecting purification and extraction efficiency in solid phase extraction (SPE) and QuEChERS methods were investigated and optimized. Matrix effects of two methods were analyzed. The results showed that the correlation coefficients were above 0.99 when the concentration was ranged from 0.01 to 0.5μg/mL. For SPE cleanup method, when the fortified levels from 0.01 to 0.5 mg/kg, the average recoveries were ranged from 67.0% to 95.6% and the relative standard deviations ( RSD) were ranged from 2.5% to 12%. For QuEChERS cleanup method, the fortified recoveries was at 0.01, 0.1 and 0.5 mg/kg were in the range of 68.2%-125%, with relative standard deviations (RSD) not more than 12.7%. All the above observations indicated that the two methods were no significant difference in sensitivity and accuracy and suitable for the determination of 19 pesticides in seedless-litchi. Besides, SPE method has the characteristics of relatively thorough purification and week matrix effect. QuEChERS cleanup is more simple, quick and green than SPE. The two methods were accurate and reliable for simultaneous determination of multiple pesticide residues in seedless-litchi.关键词
气相色谱-串联质谱(GC-MS/MS)/固相萃取(SPE)/QuEChERS/农药/残留/无核荔枝Key words
Gas chromatography-tandem mass spectrometry (GC-MS/MS)/solid phase extraction (SPE)/QuEChERS/pesticides/residue/seedless-litchi分类
农业科技引用本文复制引用
李萍萍,张月,吴小芳,阳辛凤,张振山..固相萃取法与QuEChERS法对比检测无核荔枝中19种农药残留[J].热带作物学报,2017,38(8):1565-1571,7.基金项目
海南省应用技术研发与示范推广专项(No. ZDXM2014047) （No. ZDXM2014047）
国家农产品质量安全风险评估项目(No. GJFP20170040201). （No. GJFP20170040201）
