水产学报2017，Vol.41Issue(10)：1489-1499,11.DOI:10.11964/jfc.20161010572
基于mtDNACytb基因序列的我国不同水系野生鲇种群遗传多样性与种群历史分析
Genetic diversity and population history amonggeographic populations of Silurus asotus in different water systems inChina based on mtDNA Cytb gene sequences
摘要
Abstract
The catfish,Silurus asotus, is an important commercial fish in East Asia, and it is widely distributed throughout rivers and freshwater lakes of China.Unfortunately, population quantities ofS.asotus decreased sharply in recent years because of overfishing and habitat fragmentation. Genetic diversity and population genetic structure are important aspects of the population genetics of fish, and provide essential information for understanding local adaptation and dispersal patterns, and for clarifying the genetic variation. Cytochrome b (Cytb) has a moderate evolutionary rate and a clear evolutionary pattern, suitable for the studies of phylogenetic evolution at the intra-and inter-specific levels. To identify the genetic diversity and population history among geographic populations of S.asotus in different water systems in China, including Yangtze River, Pearl River, Liao River and Southeast coastal rivers, and clarify its population demographic history and genetic structure, the genetic diversity, structure, differentiation, and molecular variance were analyzed using DNAsp5.10.01 and Arlequin 3.11. In the present study, 1110 bases of mtDNACytb gene were obtained from 216 ofS. asotus collected in the different water systems in China from 2011 to 2012. Generally, there exists high diversity between haplotype (Hd=0.948±0.009) and nucleotide (Pi=0.01799±0.00055) in all populations of the catfish, suggesting that the populations were relatively large and stable. In addition, seventy-eight haplotypes, Hap45 was shared in individuals of all populations. Phylogenetic analysis of the MEGA5 was conducted to determine the relationships betweenS.asotus haplotypes, and detect discernible groups related to geography, and these analyses distinguished four inclusive clades. Moreover, the median-joining network was similar to the topology of the phylogenetic tree with 78 haplotypes, and revealed obivously four lineages in phylogeographic pattern. The results of neutrality tests on S.asotus data set of the four lineages, combined with the nuimodal mismatch distribution, indicated recent population expansion ofS. asotus on large spatial scales in the period of late Pleistocene (0.04-0.05 Ma).关键词
鲇/细胞色素b(Cytb)/线粒体DNA/种群历史Key words
Silurus asotus/cytochrome b (Cytb)/mtDNA/population history分类
生物科学引用本文复制引用
徐丹丹,黄燕,曾庆,李斌,彭作刚..基于mtDNACytb基因序列的我国不同水系野生鲇种群遗传多样性与种群历史分析[J].水产学报,2017,41(10):1489-1499,11.基金项目
公益性行业(农业)科研专项(200903048-089) （农业）
内江师范学院校级项目(15JC12)Special Fund for Agro-Scientific Research in the Public Interest (200903048-089) （15JC12）
the Project of Neijiang Normal University (15JC12) （15JC12）
