水产学报2017，Vol.41Issue(10)：1512-1520,9.DOI:10.11964/jfc.20161010592
微卫星多重PCR分析食蚊鱼系统发育关系
Microsatellite SSR multiplexed amplification to analyze the phylogenetic relationship among invasive mosquitofish (Gambusia affinis)
摘要
Abstract
Mosquitofish(Gambusia affinis)is a globe invasive species. In order to analyze why they can invade so quickly all over the world, 11 polymorphic microsatellite loci were isolated and employed to examine paternity patterns in 755 samples of 53 families from 10 locations in 3 rivers basins. After sequence genetic analysis of the multiplex SSR PCRs, we got the best fullsib family and analyzed the phylogenetic relationship. We found that there are multiple inseminations inG. affinis. The female in the same womb can reproduce a number of the juveniles, up to 72, with different males, even up to 9. The max fullsib is 41, but the breeder's size isn't the max even not bigger than others. At the same time the max size of the breeder isn't the female which reproduce the largest number of the juveniles. The phylogenetic tree shows that the evolution was far away the sea. The 10 populations can be obviously divided into 3 clusters. The conclusion can be drawn that the key reason of the invasion isG. affinis multiple inseminations. It is suspected that the fertilized frequency isn't proportional to the size of female. Their gene flows rapidly specifically when they were in the migratory distance.关键词
食蚊鱼/多重PCR/SSR/多重授精/亲子关系评估Key words
Gambusia affinis/multiplex PCR/SSR/multiple insemination/parentage assessment分类
生物科学引用本文复制引用
刘世英,宋春高,何只翰,卢育军,吴海生,王波,曾媛琴..微卫星多重PCR分析食蚊鱼系统发育关系[J].水产学报,2017,41(10):1512-1520,9.基金项目
西南大学博士基金(swu110064)Doctoral Foundation of Southwest University(swu110064) （swu110064）
