水产学报2017，Vol.41Issue(10)：1533-1541,9.DOI:10.11964/jfc.20160310329
DNA条形码在马鞍列岛海域皮氏叫姑鱼胃含物鉴定中的应用
Application of DNA barcoding in analyzing food composition of Belanger's croaker (Johnius belangerii) in Ma'an Archipelago
摘要
Abstract
Belanger's croaker (Johnius belangerii) is a fundamental fish species of familySciaenidae which usually has high gastric vacuous rate during 24 hours trawl surveys making the diet analysis difficult to continue. However, it plays an important role in the predator-prey relationship in the food web of Ma'an Archipelago, East China Sea. In this study, we use visual inspection with DNA barcoding technology combined previous collected data to make more comprehensive analysis towards its diet compositions and realistic prey-predator relationships. We amplified mitochondrial cytochrome oxidase subunit I (COI) using fish and invertebrates universal primers in sampled fish stomach contents which could not be identified by morphological observation. Our results indicated that using DNA barcoding could successfully identify those partially digested food compositions species, such as Solenocera crassicornis,Alpheus distinguendus,Palaemon macrodactylus,Alpheus japonicus,Acetes chinensis, Sebastiscus marmoratus. We found belanger's croaker mostly feeds on shrimps (frequency of occurrence, 45%) and crabs (23%), some juvenile fishes (27%) occasionally and among themS. crassicornis,P. macrodactylus, Portunus sanguinolentus,Oratosquilla oratoria had not been observed in summer investigation ever before. In conclusion, the accurate and sufficient diet analysis is the precondition of feeding habit research which could contribute to reasonable suggestions of the management of island reef area ecosystem and fisheries forecasting.关键词
皮氏叫姑鱼/胃含物鉴定/线粒体细胞色素氧化酶亚基Ⅰ/DNA条形码/种类鉴定/摄食生态Key words
Johnius belangerii/stomach contents/CO/DNA barcoding/species identification/feeding ecology分类
生物科学引用本文复制引用
席晓晴,鲍宝龙,章守宇..DNA条形码在马鞍列岛海域皮氏叫姑鱼胃含物鉴定中的应用[J].水产学报,2017,41(10):1533-1541,9.基金项目
国家自然科学基金(41176110)National Natural Science Foundation of China (41176110) （41176110）
