水产学报2017，Vol.41Issue(10)：1542-1551,10.DOI:10.11964/jfc.20160410337
基于线粒体控制区序列的光裸方格星虫群体遗传多样性分析
Population genetic diversity analysis of Sipunculus nudus based on mtDNA control region sequences
摘要
Abstract
In order to study the population genetic structure and germplasm resources of theSipunculus nudus. mtDNA control region sequences ofS. nudus of six geographic populations along the coast of Beibu Gulf (Beihai, Zhanjiang, Qinzhou, Fangchenggang, Danzhou of China and Haiphong of Vietnam) were analyzed. Results showed that a total of 93 control region sequences with 107 variable sites and 85 haplotypes were obtained, and an evident AT-skew was found in mtDNA control region sequences ofS. nudus; the haplotype diversity (Hd) and nucleotide diversity (Pi) were 0.998 and 0.01889 respectively. The NJ (Neighbor Joining) tree base on haplotype showed that the six populations presented no obvious branches, and all haplotypes were randomly distributed in the haplotype network. The range of fixation index (Fst) of different populations was -0.01813-0.02805, and the genetic differentiation among populations was not obvious. The results of AMOVA revealed that the genetic variance mainly occurred within populations (99.74%). In terms of the negatively selective neutrality test and mismatch distribution of pairwise, we could suggest that a population expansion occurred inS. nudus, and the estimated time was about 1710000 years ago. The results indicated that there was high genetic diversity inS. nudus and existed gene exchange among populations, and the estimated population expansion could occur during the Early Pleistocene.关键词
光裸方格星虫/遗传结构/线粒体控制区/北部湾Key words
Sipunculus nudus/genetic structure/control region of mtDNA/Beibu Gulf分类
生物科学引用本文复制引用
彭银辉,周于娜,刘旭佳,黄国强,蔡小辉,潘英..基于线粒体控制区序列的光裸方格星虫群体遗传多样性分析[J].水产学报,2017,41(10):1542-1551,10.基金项目
国家自然科学基金(31160532) （31160532）
广西科学研究与技术开发计划项目(桂科攻14121006-2-1) （桂科攻14121006-2-1）
广西自然科学基金(2015GXNSFBA139081) （2015GXNSFBA139081）
广西科学院基本科研业务费(15YJ22HYS13)National Natural Foundation of China(31160532) （15YJ22HYS13）
Research Program of Science Technology Department of Guangxi Province (Gui S&T Tackle 14121006-2-1) （Gui S&T Tackle 14121006-2-1）
Natural Science Foundation of Guangxi Province of China (2015GXNSFBA139081) （2015GXNSFBA139081）
Basic Scientific Research Service Fee of Guangxi Academy of Sciences (15YJ22HYS13) （15YJ22HYS13）
