水产学报2017，Vol.41Issue(10)：1562-1570,9.DOI:10.11964/jfc.20160910536
急性胁迫对团头鲂铁稳态及其相关基因表达的影响
Effects of acute stress on iron homeostasis and iron homeostasis related genes in Megalobrama amblycephala
摘要
Abstract
To study the effects of acute stress reaction on iron concentration and iron homeostasis inMegalobrama amblycephala, we stimulated stress response by intraperitoneal injection of cortisol. The iron content in serum and liver was determined by spectrophotometer, and the iron homeostasis related gene (hepcidin & transferrin) expression were detected by quantitative real-time PCR at 0, 2, 4, 8, 10, 12, 24 and 48 h after cortisol injection. The results showed that the content of serum cortisol immediately increased significantly after injection, almost 10 folds compared with the control group, and gradually decreased backward. The content of serum iron in experimental group is higher than that in control group at 4, 8, 10 and 12 hours post injection (hpi), but the content of liver iron led to the opposite results. Hepcidin mRNA expression in liver rose remarkably, reaching the maximum at 8 hpi and 10 hpi, respectively, and then descended to the normal level. The transferrin showed similar expression pattern, and reached the peak at 10 hpi. The result indicated that large release of intracellular iron into body fluid led to increased risk of pathogenic infection and proliferationin vivo. Hepcidin and Tf were involved in regulation of iron metabolism and inflammatory response under stress inM. amblycephala and may play important roles in iron homeostasis and innate immune system.关键词
团头鲂/铁/皮质醇/铁调素/转铁蛋白/急性胁迫Key words
Megalobrama amblycephala/iron/cortisol/hepcidin/transferrin/acute stress分类
农业科技引用本文复制引用
高金伟,习丙文,滕涛,陈凯,秦婷,谢骏..急性胁迫对团头鲂铁稳态及其相关基因表达的影响[J].水产学报,2017,41(10):1562-1570,9.基金项目
国家自然科学基金(31572662) （31572662）
现代农业产业技术体系建设专项(CARS-45) （CARS-45）
江苏省水产三新工程项目(D2015-13)National Natural Science Foundation of China (31572662) （D2015-13）
Earmarked Fund for China Agriculture Research System (CARS-45) （CARS-45）
Aquaculture Research Program of Jiangsu Province (D2015-13) （D2015-13）
