水产学报2017，Vol.41Issue(10)：1571-1577,7.DOI:10.11964/jfc.20160610447
急性铅染毒对河南华溪蟹副性腺诱导精子顶体反应的影响
The effect of acute lead on accessory gland of freshwater crab (Sinopotamon henanense) in sperm acrosome reaction induction
摘要
Abstract
To investigate the toxic effect of lead on male accessory gland function in the freshwater crab Sinopotamon henanense, the function of accessory gland was investigated firstly, acrosome reaction induction rate was high up to 78% under the combination of calcium ionophore A23187 and accessory gland homogenate, it implied that accessory gland could adjust the action of sperm acrosome reaction. Then the effects of Pb on the accessory gland function in sperm acrosome reaction induction and oxidative stress were investigated after 3, 5 and 7 d, exposed to different Pb concentrations (0, 3.675, 7.35, 14.70, 29.40 and 58.80 mg/L). The results showed that the acrosome reaction induction rate was deceased significantly when the accessory gland was taken from crabs which was treated by Pb. Meanwhile, the reactive oxygen species (ROS) content and protein carbonylation (PCO) content were increased in a concentration- and time-dependent manner in accessory gland, however, DNA-protein crosslinks rate (DPC) level had no significant change. Our findings documented that Pb deceased the function of accessory gland in sperm acrosome reaction induction, which was associated with the increasing of ROS leading to protein oxidative damage.关键词
河南华溪蟹/副性腺/顶体反应/蛋白质羰基化/DNA-蛋白质交联率/铅Key words
Sinopotamon henanense/accessory gland/acrosome reaction/protein carbonylation/DNA-protein crosslink rate/lead分类
农业科技引用本文复制引用
李娜,许鹏,王兰..急性铅染毒对河南华溪蟹副性腺诱导精子顶体反应的影响[J].水产学报,2017,41(10):1571-1577,7.基金项目
国家自然科学基金(31672293, 30970361) （31672293, 30970361）
山西省应用基础研究项目(2016021141)National Nature Science Foundation of China (31672293,30970361) （2016021141）
Applied Basic Research Program of Shanxi Province (2016021141) （2016021141）
