水产学报2017，Vol.41Issue(10)：1578-1587,10.DOI:10.11964/jfc.20161010573
不同高温胁迫条件下的坛紫菜中植物激素分析
The profiling of eleven phytohormones in Pyropia haitanensis under different high-temperature environments
摘要
Abstract
In order to investigate the relationship between the high temperature resistance and variation of phytohormones under high temperature environment, thalli ofPyropia haitanensis, Zhedong No. 1, collected from Xiangshan, Ningbo were used as experimental materials. And a method using high performance liquid chromatography coupled with mass spectrometry in selective reaction mode was developed, to analyse the variations of 11 kinds of phytohormones ofP. haitanensis under different high temperature environments. The results showed that there were totally 7 kinds of phytohormones detected in theP. haitanensis under different high temperature environments, including N6-(2-isopentenyl) adenosine, N6-(2-isopentenyl)adenine,trans-zeatinriboside, indole-3-acetic acid, jasmonic acid, salicylic acid and methyl jasmonate. At the different high temperature 20, 25, 28 and 35 °C, the contents of methyl jasmonate and jasmonic acid were increased as the stimulated temperature rose. While the contents of N6-(2-isopentenyl) adenosine, N6-(2-isopentenyl) adenine, trans-zeatinriboside and indole-3-acetic acid in high temperature group were lower than those of control group and showing the decreased trends. When at the different heat shock time, as the stimulated time increased, the contents of N6-(2-isopentenyl) adenosine, N6-(2-isopentenyl)adenine,trans-zeatinriboside, indole-3-acetic acid were reduced, while the contents of salicylic acid and methyl jasmonate were increased by comparing to that of control group. However, the content of jasmonic acid was increased, then decreased at 6h to that of control group. In the recovery procedure, the contents of indole-3-acetic acid, jasmonic acid, salicylic acid and methyl jasmonate were decreased, then increased, finally decreased again. For N6-(2-isopentenyl) adenosine, N6-(2-isopentenyl)adenine andtrans-zeatinriboside, the contents of them were reduced gradually. Under high-temperature stress, the stress responses ofP. haitanensis are co-regulated by positive growth regulator phytohormones and stress phytohormones to maintain its growth. However, the defense capacity ofP. haitanensis is limited and it might be difficult for algae to repair its damage caused by high-temperature stress.关键词
坛紫菜/高温胁迫/植物激素/液质联用Key words
Pyropia haitanensis/high-temperature treatment/phytohormone/LC-MS分类
农业科技引用本文复制引用
宋悦,崔晓山,陈娟娟,杨锐,严小军..不同高温胁迫条件下的坛紫菜中植物激素分析[J].水产学报,2017,41(10):1578-1587,10.基金项目
国家星火计划项目(2015GA701027) （2015GA701027）
浙江省自然科学基金(LY17D060002) （LY17D060002）
浙江省科技厅公益项目(分析测试) (2016C37090) （分析测试）
宁波大学王宽诚幸福基金China Spark Program (2015GA701027) （2015GA701027）
Natural Science Foundation of Zhejiang Province of China (LY17D060002) （LY17D060002）
Grant of Science Technology Department of Zhejiang Province (2016C37090) （2016C37090）
K. C. Wong Magna Fund in Ningbo University （）
