山东医药2017，Vol.57Issue(39)：23-26,4.DOI:10.3969/j.issn.1002-266X.2017.39.006
双益方剂及其有效成分对胰岛素抵抗小鼠胰岛β细胞株胰岛素分泌、增殖、凋亡的影响
Effects of Shuangyi decoction and its active ingredients on insulin secretion proliferation and apoptosis of IR mouse pancreatic β cell strain
摘要
Abstract
Objective To observe the effects of Shuangyi decoction (SY)and its effective ingredients on the insulin secretion,cell proliferation,and apoptosis of islet β cells Min 6 in insulin resistant (IR)mice,and to analyze the action mechanism. Methods We cultured Min6 cells and then divided them into the normal group,IR model group,metformin group,SY group,single herb of SY group [Cornus officinalis (Shanzhuyu),Astragalus membranaceus (Huangqi),Coptis chinensis (Huanglian),Puerarin (Gegen),Cortex mori radicis (Sangbaipi),and Eupatorium (Peilan)],and SY effec-tive ingredient group (1-deoxynojirimycin,berberine,astragaloside,ursolic acid,loganin,calycosin glucoside,and puera-rin). The normal group was cultured with phenol red free medium;the other groups were added with glucose and palmitic acid in order to make the Min6 cell IR models. Metformin group was added with 100 g/ L metformin. SY group,Shanzhuyu group,Huangqi group,Huanglian group,Gegen group,Sangbaipi group,Peilan group,1-deoxynojirimycin,berberine, astragaloside,ursolic acid,loganin,calycosin glucoside,puerarin groups were added with 50,100,and 200 g/ L the cor-responding drugs. Insulin secretion test was performed at 24 h after administration,and the level of insulin secretion was detected. The proliferation ability of cells at 24 h and 48 h after administration was detected by CCK-8 kit. Apoptotic cells were detected at 24 h after administration by double staining in the normal group,IR model group,metformin group,and SY group;the apoptotic rate was also calculated;the protein exoression of ERK1 / 2,p-ERK1 / 2,MEK1 / 2 was detected by Western blotting. Results Under the condition of high glucose and low sugar,the insulin secretion of the IR model group was lower than that of normal group (P < 0. 05). The insulin secretion levels of the metformin group,Huangqi group and Gegen group,SY group,astragaloside group,Shanzhuyu group,1-deoxynojirimycin group,puerarin group,and berberine group were higher than that of the IR model group (all P < 0. 05). The cell proliferation ability of IR model group was low-er than that of the normal group (P < 0. 05);the cell proliferation abilities of the metformin group,SY group,Huangqi group,astragaloside group,1- deoxynojirimycin group,and Shanzhuyu group were higher than that of IR model group (P <0. 05). The apoptosis rate of the IR model group was higher than that of the normal group,and the apoptosis rate of the SY group was lower than that of the IR model group (P < 0. 05). The expression levels of MEK1 / 2,ERK1 / 2,and p-ERK1 /2 proteins were lower in the IR model group than in the normal group (P < 0. 05);the expression levels of MEK1 / 2, ERK1 / 2,and p-ERK1 / 2 proteins were higher in the metformin group and SY group than in the IR model group (all P <0. 05). Conclusions SY,and its effective ingredients Huangqi,Gegen,astragaloside,Shanzhuyu,1-deoxynojirimycin, puerarin,and berberine can effectively improve the IR Min6 cell insulin secretion,promote Min6 cell proliferation and in-hibit the apoptosis by up-regulating the expression of ERK1 / 2,p-ERK1 / 2 and MEK 1 / 2 protein.关键词
双益方/2型糖尿病/胰岛素抵抗/胰岛素分泌实验/细胞增殖/细胞凋亡/小鼠胰岛β细胞株Key words
Shuangyi decoction/type 2 diabetes mellitus/insulin resistance/insulin secretion test/cell prolifera-tion/apoptosis/mouse pancreatic β cell strain分类
医药卫生引用本文复制引用
田春雨,马雷雷,喇孝瑾..双益方剂及其有效成分对胰岛素抵抗小鼠胰岛β细胞株胰岛素分泌、增殖、凋亡的影响[J].山东医药,2017,57(39):23-26,4.基金项目
河北省自然科学基金资助项目(H2015209025) （H2015209025）
河北省高等学校科学技术研究项目(QN2015119). （QN2015119）
