山东医药2017，Vol.57Issue(39)：27-29,3.DOI:10.3969/j.issn.1002-266X.2017.39.007
高糖高脂、PAF对肾小球系膜细胞PKCβI mRNA表达的影响及阿托伐他汀干预作用观察
Effects of high glucose and lysophosphatidylcholine and PAF on PKCβI mRNA expression of glomerular mesangial cells and the intervention effect of atorvastatin
摘要
Abstract
Objective To investigate the effect of atorvastatin on the PKCβI mRNA expression of high glucose and lysophosphatidylcholine (LPC)and platelet activating factor (PAF)-induced mesangial cells. Methods Human glomeru-lar mesangial cells (HMCs)were divided into four groups:the normal control group (group A),high glucose and LPC +PAF group (group B),high glucose and LPC + PAF + LY333531 group (group C),and high glucose and high LPC +PAF + atorvastatin group (group D). We added 5. 5 mmol/ L D-glucose to group A;group B was treated with 30 mmol/ L D-glucose,20 mg/ L LPC,and 2 × 10 - 8 mol/ L PAF C-16;group C was pretreated with 2 × 10 - 7 mol/ L LY333531 for 1 h, and then was added with 30 mmol/ L D-glucose,20 mg/ L LPC,and 2 × 10 - 8 mol/ L PAF C-16;group D was pretreated with 10 μmol/ L atorvastatin for 1 h,and then was added with 30 mmol/ L D-glucose,20 mg/ L LPC,2 × 10 - 8 mol/ L PAF C-16. At 24 h after culture,RNA was extracted. The expression of PKCβI mRNA in the mesangial cells was detected by real-time fluorescence quantitative PCR. Results The relative expression of PKCβI-mRNA in the groups A,B,C,and D was 1. 00 ± 0. 00,3. 59 ± 0. 41,2. 76 ± 0. 57,and 1. 02 ± 0. 00,respectively. The expression of PKCβI-mRNA in the group B group was higher than that of the other three groups,and the expression of PKCβI-mRNA in the group D was sig-nificantly lower than that of the groups B and C (all P < 0. 05). Conclusions The expression of PKCβI mRNA in high glucose and LPC and PAF-induced glomerular mesangial cells is up-regulated,and atorvastatin can inhibit the PKCβI ex-pression.关键词
肾小球系膜细胞/血小板活化因子/蛋白激酶C-βI/阿托伐他汀Key words
glomerular mesangial cells/platelet-activating factor/protein kinase C-βI/atorvastatin分类
医药卫生引用本文复制引用
肖艳华,何笑云,韩晴,胡小文,周素娴..高糖高脂、PAF对肾小球系膜细胞PKCβI mRNA表达的影响及阿托伐他汀干预作用观察[J].山东医药,2017,57(39):27-29,3.基金项目
国家自然科学基金资助项目(81560148,81260134). （81560148,81260134）
