上海海洋大学学报2017，Vol.26Issue(5)：674-681,8.DOI:10.12024/jsou.20170502052
罗氏沼虾5个专门化品系选择系繁殖力的比较分析
Comparative analysis of fecundity of 5 specialized strains of shrimp Macrobrachium rosenbergii
摘要
Abstract
In order to investigate the breeding performance of Macrobrachium rosenbergii,this experiment was conducted by measuring 5 specialized strains of shrimp Macrobrachium rosenbergii (A,B,C,D,E) of the three consecutive spawning unit weight of fecundity (Fw) ovarian development cycle (Co),fertilized egg diameter (DE),vitellogenin (VTG) content,and cathepsin D (CTSD) activity index,and comparative analysis of 5 selection strains of fertility was made,with a view to screening the high fecundity strains of Macrobrachium rosenbergii.The results showed that the absolute fecundity (F),Fw and body weight were positively correlated (P ＜ 0.05),F and Fw were also significantly positively correlated (P ＜ 0.01).The average value of Fw of the continuous thrice spawning of the 5 selected strains was (1.96-2.30) × 103 eggs/ g,the differences between the strains were significant (P ＜ 0.05).The Fw of B and D were 2.26 × 103 eggs/ g and 2.30 × 103 eggs/g respectively.The average range of VTG content and CTSD activity in the 5 selected strains were 3.48-5.80 μg/g,271.81-320.82 U/g,and there was significant difference among the strains (P ＜ 0.05),while the highest content of VTG in fertilized eggs was B (5.80 μg/g),the highest CTSD activity was D (320.82 U/g),the second was B (303.09 U/g),and there was no difference between them (P ＞0.05).The range of Co was 18.8-23.8 d,second time larger than the first (2-8 d),there was no difference(P ＞ 0.05) between each strain,which were all significantly lower than those in the control group (P ＜ 0.05).The mean value range of DE was 542.3-552.0 nm,there is no difference among strains,and the average egg diameters of B and D were larger,551.7 nm and 552.0 nm,respectively.It can be confirmed that among 5 specialized strains of Macrobrachium rosenbergii,the fecundity of B and D is the strongest,A is weak,C and E is the weakest,which can provide basis for breeding of specialized strains of shrimp Macrobrachium rosenbergii.关键词
罗氏沼虾/专门化品系/繁殖力/卵黄蛋白原/组织蛋白酶DKey words
Macrobrachium rosenbergii/specialized strains/fecundity/vitellogenin/cathepsin D分类
农业科技引用本文复制引用
袁新程,戴习林,朱其建,李玉峰,胡彦杰,周迅,丁福江..罗氏沼虾5个专门化品系选择系繁殖力的比较分析[J].上海海洋大学学报,2017,26(5):674-681,8.基金项目
上海市现代农业产业技术体系建设项目(沪农科产字[2016]第5号) （沪农科产字[2016]第5号）
