上海海洋大学学报2017，Vol.26Issue(5)：682-690,9.DOI:10.12024/jsou.20170301999
稻蟹共作与蟹单作模式下中华绒螯蟹肠道及养殖环境细菌群落组成比较
A comparative study of microbiota from the intestine of Chinese mitten crab (Eriocheir sinensis) and their culture environment, between rice-crab co-culture and crab monoculture models
摘要
Abstract
To investigate the differences in the compositon and diversity of bacterial community in the intestine,water and sediment of rice-crab co-culture and crab monoculture models,Illumina sequencing of the V4 hypervariable region 16S rRNA gene revealed extensive changes in bacterial communities.At the phylum level,7 bacterial phyla were found,including Acidobacteria and Synergistetes in the intestine,Bacteroidetes,Cyanobacteria,Gemmatimonadetes and Armatimonadetes in the water,Planctomycetes in the sediment.At the genus level,there were 14,18 and 13 bacterial genera in the intestine,water and sediment respectively.No significant differences in the abundance indexes (Chao,ACE) and the diversity indexes (Simpson,Shannon) of the intestinal,water and sediment samples were observed between two production models.Comparative analysis of bacterial groups with significant difference in the water and sediment (Mycobacterium,Rhodobacter,Flavobacterium,Nitrospira,Cyanobacteria,Acidobacteria,Planctomycetes,Tenericutes and Firmicutes) revealed bacterial species that indicate an anaerobic or high nitrite environment was more abundant in the samples of crab monoculture model.The composition of intestinal bacterial community of ricecrab co-culture model was closer to that in wild populations,which also indicated the environment of rice-crab co-culture is healthier than crab monoculture and is beneficial to the growth of Eriocheir sinensis.关键词
中华绒螯蟹/细菌群落/稻蟹共作/肠道/环境Key words
Eriocheir sinensis/bacterial community/rice-crab/intestine/environment分类
农业科技引用本文复制引用
成永旭,刘洪天,常东,曾奇韬,孙中勇,李嘉尧..稻蟹共作与蟹单作模式下中华绒螯蟹肠道及养殖环境细菌群落组成比较[J].上海海洋大学学报,2017,26(5):682-690,9.基金项目
上海高校水产学高峰学科建设项目(2015-62-0908) （2015-62-0908）
上海科技兴农推广项目(沪农科推字2015第1-7号) （沪农科推字2015第1-7号）
“稻-渔”耦合养殖技术研究与示范(201203081) （201203081）
科技部港澳台科技合作专项(2014DFT30270) （2014DFT30270）
