生物骨科材料与临床研究2017，Vol.14Issue(5)：5-8,4.DOI:10.3969/j.issn.1672-5972.2017.05.002
复合磷酸钙颗粒溶解性能测试研究
The study on dissolution testing of Calcium phosphate composite particles
摘要
Abstract
Objective To study the dissolution characteristics of calcium phosphate composite particles (HA ∶ TCP=3:7) in TRIS buffer solution.Methods A series of standard Ca2+solutions with increasing concentration are prepared,and the working curve of Ca2+ ion solution was drawn.The composite calcium phosphate particles (HA ∶ TCP=3:7) in the pH value for 7.4 TRIS buffer solution,sampling once per hour (initial samples were collected every 3 min),extracted from the supematant by high speed centrifugation,measured at each time point of the concentration of Ca2+ extraction liquid by inductively coupled plasma mass spectrometry.The dissolution curve of Ca2+ concentration on soaking time was drawn.Results ① The linear equation of the working curve of Ca2+ ionic solution is y-4043.9x+23783,and the correlation coefficient R is 0.9999.The detection limit is 0.0063 μg/mL,and the quantitative limit is 0.021 μg/mL.② After the initial immersion within 0.5 h,the concentration of Ca2+ increased with time dramatically,marked a turning point at 0.5 h,From 0.5 h to 2 h,the concentration of Ca2+ ion increased with time,but the rate of increase decreased obviously compared with the first 0.5 h.After20h,the dissolution reached the basic balance.③Thedissolutionprocessofcalcium phosphate composite particles can be divided into 3 stages,the first is the dissolution process of Ca2+ ion concentration increased rapidly,the second stage is the competition process of Ca2+ dissolution with HA or CaHPO4 · 2H2O deposition,the third stage is the stable process of Ca2+ ion concentration will no longer increase.Conclusion The dissolution of composite calcium phosphate particles (HA ∶ TCP=3 ∶ 7) in TRIS buffer solution has a certain regularity,and the concentration of Ca2+ ions dissolved in 24h is about 1200 μg/L.关键词
磷酸钙/溶解行为/电感耦合等离子体质谱法Key words
Calcium phosphate/Dissolution behavior/ICP-MS分类
医药卫生引用本文复制引用
马春宝,李沅,景明,樊铂..复合磷酸钙颗粒溶解性能测试研究[J].生物骨科材料与临床研究,2017,14(5):5-8,4.基金项目
国家科技支撑计划课题“战略性新兴医疗器械产业关键技术标准研究”(课题编号:2015BAI44H01) （课题编号:2015BAI44H01）
