实用肿瘤杂志2017，Vol.32Issue(5)：411-415,5.
TAGLN基因过表达对乳腺癌细胞迁移和侵袭能力的影响
Effects of TAGLN overexpression on the migration and invasion of breast cancer cell
摘要
Abstract
Objective To investigate the effects of TAGLN overexpression on the migration and invasion of human breast cancer cell MDA-MB-231 and the molecular mechanism.Methods Lentiviral expression system was used in MDA-MB-231 cells to construct the TAGLN stable overexpression cell lines.MDA-MB-231 cells untreated were used as blank control group and those stably transfected with empty vector lentivirus were used as control group.The expression of TAGLN mRNA and protein were detected by real time PCR and Western blot.Wound scratch assay and transwell invasion assay were carried out to determine the migration and invasion ability of cells.Western blot was applied to determine the expression levels of matrix metalloproteinase-2 (MMP-2) and MMP-9 after TAGLN overexpression.Results After the infection of TAGLN overexpression lentivirus vector in MDA-MB-231 cells,the expression of TAGLN mRNA and protein were signi ficantly increased (both P ＜ 0.01),which suggested the successful construction of TAGLN stable overexpression cell lines (231-TAGLN).The in vitro migration and invasion ability of 231-TAGLN cells were decreased significantly,compared with the control group and blank control group (all P ＜ 0.01).Moreover,the expression levels of MMP-2 and MMP-9 were both decreased in 231-TAGLN cells (both P ＜ 0.01).Conclusion TAGLN overexpression significantly inhibits the migration and invasion of breast cancer cells,and the downregulation of MMP-2 and MMP-9 expression may be involved in this process.关键词
乳腺肿瘤/病理学/微丝蛋白质类/转录,遗传/基因表达/基质金属蛋白酶2/代谢/基质金属蛋白酶9/代谢/细胞运动/肿瘤侵润/聚合酶链反应Key words
breast neoplasms/pathology/microfilament proteins/transcription, genetic/gene expression/matrix metalloproteinase 2/metabolism/matrix metalloproteinase 9/metabolism/cell movement/neoplasm invasiveness/polymerase chain reaction分类
医药卫生引用本文复制引用
杨彧,付雯..TAGLN基因过表达对乳腺癌细胞迁移和侵袭能力的影响[J].实用肿瘤杂志,2017,32(5):411-415,5.基金项目
成都中医药大学科技发展基金资助项目(ZRQN1419) （ZRQN1419）
四川省科技厅基金资助项目(2017JY0208) （2017JY0208）
