特产研究2017，Vol.39Issue(3)：21-27,7.DOI:10.16720/j.cnki.tcyj.2017.03.005
狐源豚鼠气单胞菌M12胞外产物特性研究
Characterization of Extracelluar Production of Aeromonas Caviae Named M12 from Fox
摘要
Abstract
After the extracelluar production was extracted and concentrated from M12,the protein content,major components and toxicity of extracellular product (ECP) to Vero cells were detected.Pathogenicity of ECP was also researched by intramuscular injection and intestinal loop test.The results showed that the ECP's concentration is 0.67μg/mL and contained the esterase,protease,amylase and gelatinase with urease activity;ECP of M12 had acytotoxic effect on Vero cells and enterotoxin activities to intestinal tract;ECP was sensitive to temperature (optimum temperature is 37℃) and pH (optimum pH is 7.0);SDS-PAGE of ECP of M12 showed three proteins bands (36ku,42ku,and 68ku);The muscle tissue of the rabbits was observed to dissolve in leg followed by injection of ECP;the contents of intestine increased significantly after injection of ileum.In conclusion.M12 had many virulent factors with close relationship to its pathogenicity.关键词
胞外产物/细胞毒性/豚鼠气单胞菌/肠攀试验Key words
extracelluar production/cytotoxicity/Aeromonas caviae/intestinal loop test分类
农业科技引用本文复制引用
孟藏龙,陈立志..狐源豚鼠气单胞菌M12胞外产物特性研究[J].特产研究,2017,39(3):21-27,7.基金项目
毛皮动物重要传染病快速诊断体系的建立(20130077) （20130077）
