超高效液相色谱-串联质谱法测定松花粉中赤霉素的含量OACSTPCD
Determination of Gibberellin in Massoniana Pollen by UPLC/Q-TOF/MS
建立了超高效液相色谱-串联质谱法测定松花粉中赤霉素的含量.松花粉提取物经80％乙醇提取、正丁醇萃取后,采用BEH-C18色谱柱分离,乙腈和水作为流动相进行梯度洗脱.结果表明,松花粉中赤霉素含量为0.075mg/g,在50μg/mL～250μg/mL的范围内线性关系良好(r=0.9994,n=3),平均回收率为92.2％ (RSD％=1.5,n=3).本方法操作简便、灵敏度高、稳定性好,为松花粉中赤霉素的含量测定提供了一种新的色谱技术方法.
A method was established for determination Gibberellin in Massoniana Pollen by UPLC/Q-TOF/MS.Samples were extracted with 80％ ethanol,and were extracted by butyl alcohol.The target analytes were separated on a BEH-C18 column with gradient elution using a mobile phase made up of acetonitrile and water.The result showed that contents of Gibberellin in Pius Massoniana Pollen is 0.075mg/g,The linearities of Gibberellin were in the concentration range of50μg/mL-25…查看全部>>
李伟;尹建元;石丽花;王春红;曲涛;李亨;杨长军;吴迪;王桐;范秀双
吉林省中医药科学院,长春130000吉林大学药学院,长春130021烟台新时代健康产业有限公司,山东烟台264000吉林大学药学院,长春130021烟台新时代健康产业有限公司,山东烟台264000吉林大学药学院,长春130021烟台新时代健康产业有限公司,山东烟台264000吉林大学药学院,长春130021烟台新时代健康产业有限公司,山东烟台264000吉林大学药学院,长春130021
医药卫生
超高效液相色谱-串联质谱法松花粉赤霉素含量测定
UPLC/Q-TOF/MSmassoniana Pollengibberellincontent determination
《特产研究》 2017 (3)
28-32,5
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