特产研究2017，Vol.39Issue(3)：28-32,5.DOI:10.16720/j.cnki.tcyj.2017.03.006
超高效液相色谱-串联质谱法测定松花粉中赤霉素的含量
Determination of Gibberellin in Massoniana Pollen by UPLC/Q-TOF/MS
李伟 1尹建元 2石丽花 3王春红 2曲涛 3李亨 2杨长军 3吴迪 2王桐 3范秀双2
作者信息
- 1. 吉林省中医药科学院,长春130000
- 2. 吉林大学药学院,长春130021
- 3. 烟台新时代健康产业有限公司,山东烟台264000
- 折叠
摘要
Abstract
A method was established for determination Gibberellin in Massoniana Pollen by UPLC/Q-TOF/MS.Samples were extracted with 80％ ethanol,and were extracted by butyl alcohol.The target analytes were separated on a BEH-C18 column with gradient elution using a mobile phase made up of acetonitrile and water.The result showed that contents of Gibberellin in Pius Massoniana Pollen is 0.075mg/g,The linearities of Gibberellin were in the concentration range of50μg/mL-250μg/mL(r=0.9994,n=3),the average recovery was 92.2％(RSD％=1.5,n=3).Providing a new chromatographic technology for the content analysis of Gibberellin in the Massoniana Pollen,the method was proved to be simple,convenient,sensitive and good stability.关键词
超高效液相色谱-串联质谱法/松花粉/赤霉素/含量测定Key words
UPLC/Q-TOF/MS/massoniana Pollen/gibberellin/content determination分类
医药卫生引用本文复制引用
李伟,尹建元,石丽花,王春红,曲涛,李亨,杨长军,吴迪,王桐,范秀双..超高效液相色谱-串联质谱法测定松花粉中赤霉素的含量[J].特产研究,2017,39(3):28-32,5.
