Abstract
The ICP-MS method was established firstly for fast and accurate determination of essential trace elements contents in stem leaf and root of Taraxacum mongolicum.The comparison of the contents of trace elements Mn,Zn,Sr,Rb,B,Cu,Cr,Ni,V,Co,Se,Mo,existed in above-ground part and undergr-ound part of T.mongolicum was studied.This will provide theoretical basis for the medical value evaluation and the selective development of this pla-nts.Adopting the method of electric heating digestion,shoot and root powder (200mesh sieve) were digested respectively with the mixed volume ratio of 1:2 HNO3-H2O2 system,the contents of trace elements were determined by ICP-MS.In addition,In and Rh were regarded as internal standard,and the contents of trace elements were calculated by standard curve method.This method showed good linearity within the range from 0.05μg/L-150μg/L,he linear correlation coefficients (r) were greater than 0.999,the detection limit of each element were in the range of 0.008μg/g-0.02μg/g,and the reco-veries were between 96.1％ and 103.8％ respectively.Both of the shoot and root of T.mongolicum were rich in these 12 kinds of trace elements,including 4 kinds of elements Mn、Sr、Rb、B contents in stem leaf (120.70μg/g,92.88μg/g,30.69μg/g,32.67μg/g) were significantly higher than in root (81.72μg/g,36.10μg/g,20.14μg/g,14.19μg/g).Moreover,the contents of Zn、Cu、Cr、Ni、V、Co、Se、Mo in root (81.88μg/g,15.92μg/g,20.24μg/g,6.372μg/g,4.907μg/g,1.698μg/g,1.575μg/g,1.8μg/g) were higher than in shoot (70.5μg/g,12.47μg/g,8.142μg/g,5.801μg/g,3.216μg/g,1.332μg/g,1.478μg/g,1.436μg/g).The establis-hed method of ICP-MS was validated with good precision,repeatability and accuracy,which is appropriate for simultaneous determination of 12 kinds essential trace elements existed in stem leaf and root of T.mongolicum.The contents of trace elements in shoot and root were different,which will pro-vide data support for the rational use of different parts of T.mongolicum.关键词
蒲公英/茎叶/根/电感耦合等离子体质谱法/人体必需微量元素Key words
Taraxacum mongolicum Hand.-Mazz./shoot/root/ICP-MS/essential trace element分类
医药卫生
