天然产物研究与开发2017，Vol.29Issue(9)：1470-1474,5.DOI:10.16333/j.1001-6880.2017.9.003
荫蔽信号对大豆幼苗异黄酮合成的影响
Effect of Shading Signal on Isoflavone Biosynthesis of Soybean Seedling
摘要
Abstract
The reduction in the ratio of red light (R) to far-red light (FR) is an important signal of shade stress.In the present study,an experiment was conducted to study the effect of normal and low R/FR irradiation on the isoflavone contents of two soybean varieties:high shade-tolerance species'ND12'and low shade-tolerance species'C103'.Relative expression levels of key genes,which encoded isoflavone synthetic enzymes in leaves,hypocotyls and roots were also studied.The results indicated that compared with seedlings under normal light,low R/FR increased total contents of isoflavone and genistein significantly in the leaves of'ND12',but decreased in hypocotyls;while the opposite result was obtained in'C103'.Expression of isoflavone synthesis key genes,including IFS2,4CL,C4H and CHI correlated with isoflavone accumulation under low R/FR irradiation.Shading signal induced isoflavone accumulation particularly in leave of shade-tolerance varieties'.This study preliminary clarified the shading signal (low R/FR) that how to control the rules of isoflavone accumulation in soybean seedlings.This study laid a foundation to research regulation mechanism of soybean isoflavone response to shading signal.关键词
荫蔽/大豆/异黄酮/代谢/基因表达Key words
shading/soybean/isoflavone/metabolism/gene expression分类
农业科技引用本文复制引用
秦雯婷,丰宇瑞,雷震,杨才琼,吴海军,Nasir Iqbal,杨文钰,刘江..荫蔽信号对大豆幼苗异黄酮合成的影响[J].天然产物研究与开发,2017,29(9):1470-1474,5.基金项目
国家自然科学基金青年科学基金项目(31401329) （31401329）
中国博士后科学基金面上项目(2014M560724) （2014M560724）
