天然产物研究与开发2017，Vol.29Issue(9)：1498-1503,6.DOI:10.16333/j.1001-6880.2017.9.008
二烯丙基二硫对IL-1β诱导SD大鼠关节软骨细胞的影响
Effects of Diallyl Disulfide on SD Rat Articular Cartilage Cell Induced by IL-1β
摘要
Abstract
To explore the effect of diallyl disulfide (DADS) on articular cartilage cell induced by IL-1β in SD rats.The growth inhibition of articular cartilage cell induced by IL-1β in SD rats following DADS treatment was measured by CCK8 assay and plate colony formation assay;The protein expression of MMP13,TIMP1 and Collagen Ⅱ were determined using Western blot.DADS (12.5 and 25 μmol/L) significantly increased the cell proliferation and high colony formation capacity;DADS (50 and 100 μmol/L) significantly suppressed the cell proliferation and lowered colony formation capacity (P ＜ 0.05);Western blot demonstrated DADS markedly down-regulated the expression of MMP-13,and increased the expression of Collagen Ⅱ and TIMP1 in the cells.These results indicated that DADS had two-way regulation effect on SD rat articular cartilage cell induced by IL-1β,DADS (25 μmol/L) had a protective effect on SD rat articular cartilage cell induced by IL-1β,DADS (50 and 100 μmol/L) can inhibit articular cartilage cell by IL-1β in SD rats,the protection molecular mechanism was possibly related to down-regulation of the expression of MMP-13 by DADS,and increased the expression of Collagen Ⅱ and TIMP1 on SD rat articular cartilage cell with osteoarthritis.关键词
二烯丙基二硫/IL-1β/SD大鼠关节软骨细胞/双向调节Key words
diallyl disulfide/IL-1β/articular cartilage cell/two-way regulation分类
医药卫生引用本文复制引用
王宏强,仇成凤,邓紫薇,史志华,李峰,谭力铭..二烯丙基二硫对IL-1β诱导SD大鼠关节软骨细胞的影响[J].天然产物研究与开发,2017,29(9):1498-1503,6.基金项目
湖南省教育厅科研计划(14C0915) （14C0915）
湖南省中医药科研计划(201585) （201585）
湖南省卫生科研计划(B2015-181) （B2015-181）
