水土保持学报2017，Vol.31Issue(5)：350-355,6.DOI:10.13870/j.cnki.stbcxb.2017.05.055
镰刀菌-淀粉-苜蓿对煤矿区污染土壤HMW-PAHs的修复
Study on Remediation of HMW-PAHs Contaminated Soil by Fusarium sp.-Starch-Alfalfa in Coal Mine Area
摘要
Abstract
The pot experiment was conducted to investigate how alfalfa,Fusarium sp.ZHH2,starch as well as their combination of different size could remove high molecular weight PAHs (HMW-PAHs) in agricultural soil in a coal mine area.The results showed that the removal rate of 6 types of individual HMW-PAHs (BbF,BkF,BaP,DbA,InP and BghiP) was in the range of 4.22％～45.01％ in alfalfa treatment (M).There were significant differences among the combined treatments using different inoculum size of ZHH2 and alfalfa (H1 +M,H2+M,H3+M) for the degradation of HMW-PAHs.And the range of removal rates of 6 types of HMW-PAHs were 11.01％～ 45.30％ and 15.20％～43.58％ for treatment H1+M and H2+M,respectively.Compared with M treatment,the removal rates of BkF in teatment H1+ M and H2+M increased by 1.62 times and 2.60 times respectively,whereas the other PAHs had no significant difference.However,compared to treatment M,H3+M treatment had a significant negative effect on the removal of HMW-PAHs.On the basis of H and M combination,starch of different size was added to improve the removal of these HMW-PAHs in soil.Further,the effect of the three factors treatments (D1+H1+M,D1 +H2+M,D1+H3+M,D2 +H1 +M,D2+H2+M and D2+H3+M) on HMW-PAHs removal was analyzed.The results showed that the removal rates of BbF,BkF,BaP,InP,DbA and BghiP in the D1 + H2 + M treatment were the highest among all treatments of three factor combinations,which were 28.87％,54.59％,47.04％,65.91％,66.42％,and 71.88％ respectively.And there was no significant difference between D1 +H1 +M and D1 +H2 +M in the removal of each HMW-PAH.Compared with H2 +M treatment,the removal rate in the D1+H2+M treatment increased significantly by 0.46,2.59,1.71,1.15,0.52,and 1.37 times,respectively (P＜ 0.05).Therefore,the combination of Fusarium sp.ZHH2,starch and alfalfa offers a suitable alternative for phytoremediation of aged PAH-contaminated soil in coal mining areas,with a recommended inoculation size of 0.5 g Fusarium sp.ZHH2,0.25 g starch per kg soil with the growth of alfalfa.关键词
农田土壤/高环芳烃污染/紫花苜蓿/镰刀菌/淀粉Key words
farmland soil/Fusarium sp.ZHH2/alfalfa/starch/high molecular weight PAHs分类
资源环境引用本文复制引用
张丽秀,李岩,李橙,赵欧亚,石维,冯圣东,杨志新..镰刀菌-淀粉-苜蓿对煤矿区污染土壤HMW-PAHs的修复[J].水土保持学报,2017,31(5):350-355,6.基金项目
河北省人力资源和社会保障厅项目(C2013003022) （C2013003022）
河北省教育厅项目(Z2013058) （Z2013058）
