中国组织工程研究2017，Vol.21Issue(30)：4769-4774,6.DOI:10.3969/j.issn.2095-4344.2017.30.003
含锌镁合金对人成骨肉瘤U2OS细胞株体外增殖的影响
Effects of zinc-containing magnesium alloys on proliferation of human osteosarcoma U2OS cells in vitro
赵小魁 1何保华 1邵楠 1张胜国 1杨万石1
作者信息
摘要
Abstract
BACKGROUND: Alloying can improve the corrosion resistance and slow the degradation of pure magnesium. In addition, increasing studies have shown that zinc has good antitumor effect. OBJECTIVE: To evaluate the effect of zinc-containing magnesium alloy on the proliferation and apoptosis of human osteosarcoma U2OS cells in vitro.METHODS: The corrosion resistance of pure magnesium and different zinc-containing (2%, 4%, 6%) magnesium alloys were observed and compared by the hydrogen release assay in the Hank's solution. MTT assay was used to examine the effects of different zinc-containing magnesium alloy extractions on the proliferation of U2OS cells (or MC3T3-E1 cells) after co-culture of 1, 3, 5 days. After 24-hour co-culture with pure magnesium, different zinc-containing magnesium alloys and titanium alloy extractions, the apoptotic rates of U2OS cells were analyzed by flow cytometry, and the expression of apoptosis-related proteins were detected by western blot assay.RESULTS AND CONCLUSION: The corrosion resistance of pure magnesium was improved after addition of Zn within the initial 100 hours, and the magnesium alloy containing 4% zinc exhibited the optimal corrosion resistance. Different zinc-containing magnesium alloy extractions obviously inhibited the U2OS proliferation in a zinc level-depended manner, and the cytotoxicity of different zinc-containing magnesium alloy extractions to MC3T3-E1 was graded 0-1. Different zinc-containing magnesium alloy extractions could also induce obvious apoptosis in U2OS cells in a zinc level-depended manner. Different zinc-containing magnesium alloy extractions, especially the magnesium alloy containing 4% zinc, up-regulated the expression of p53 and Bax proteins and down-regulated the expression of Bcl-2 protein in U2OS cells, leading to the disorder of Bcl-2/Bax. These findings suggest that different zinc-containing magnesium alloy extractions can inhibit the proliferation and induce apoptosis of U2OS cells in vitro.关键词
生物材料/骨生物材料/镁锌合金/人成骨肉瘤U2OS细胞/细胞增殖/细胞凋亡分类
医药卫生引用本文复制引用
赵小魁,何保华,邵楠,张胜国,杨万石..含锌镁合金对人成骨肉瘤U2OS细胞株体外增殖的影响[J].中国组织工程研究,2017,21(30):4769-4774,6.
