中国组织工程研究2017，Vol.21Issue(30)：4793-4798,6.DOI:10.3969/j.issn.2095-4344.2017.30.007
Cultispher微载体构建组织工程软骨修复关节软骨缺损
Tissue-engineered cartilage construction by Cultispher microcarrier for articular cartilage repair
常彬 1肖统光2
作者信息
- 1. 长治医学院附属和平医院骨科,山西省长治市 046000
- 2. 解放军总医院骨科,北京市 100853
- 折叠
摘要
Abstract
BACKGROUND: Both Cultispher microcarrier and fibrin can act as carriers in cartilage tissue engineering, but their application is limited by poor mechanical properties and poor plasticity.OBJECTIVE: To combine Cultispher microcarrier carrying chondrocytes with fibrin glue to construct Gultispher/fibrin composite scaffold, and to investigate the effect of this composite scaffold in the articular cartilage repair in a rabbit model.
METHODS: Rabbit chondrocyte and Cultispher microcarriers were co-cultured in a stirred bioreactor until the chondrocytes adhered to and proliferated quickly on the microcarrier surface. Chondrocytes-seeded microcarries were then combined with fibrin glue to construct microcarrier/fibrin glue composite scaffolds, to repair trochlear cartilage
defects of the knee joint in the rabbit model. In the experiment, three different treatments were respectively done for repair of cartilage defects, including implantation of chondrocytes-seeded microcarries/fibrin glue composite scaffold (MCF group), implantation of Gultispher/fibrin composite scaffold (MF group), and no treatment (blank control group). At 3 and 6 months after surgery, gross observation, histological evaluation, pathological evaluation and Micro-CT scanning were conducted to evaluate the cartilage repair effects. RESULTS AND CONCLUSION: Gross observation showed that the MCF group achieved better effect on cartilage repair, compared to the other two groups. Histopathological evaluation revealed hyaline-like cartilage tissues in the MCF group while fibrocartilage tissues were seen in the other two groups, shown by safranin O staining and sirius red staining.Micro-CT scanning results showed better subchondral bone remodeling was found in the MCF group than the other two groups. Gross observation and pathological observation showed better outcomes in the MCF group than the MF and blank control groups. To conclude, the chondrocyte-seeded Cultispher microcarrier/fibrin glue composite scaffold succeeds in the articular cartilage repair.关键词
生物材料/软骨生物材料/微载体/纤维蛋白胶/软骨组织工程/软骨损伤修复分类
医药卫生引用本文复制引用
常彬,肖统光..Cultispher微载体构建组织工程软骨修复关节软骨缺损[J].中国组织工程研究,2017,21(30):4793-4798,6.
