中国组织工程研究2017，Vol.21Issue(30)：4873-4878,6.DOI:10.3969/j.issn.2095-4344.2017.30.020
胶原蛋白海绵对肝脏创伤止血的效果
Hemostatic effect of collagen sponge on liver trauma
张自强 1张以河 1祖耀先 2安琪 1徐荣荣3
作者信息
- 1. 非金属矿物与固废资源材料化利用北京市重点实验室,中国地质大学(北京)材料科学与工程学院,北京市 102200
- 2. 北京市红十字会急诊抢救中心,北京市 102200
- 3. 北京湃生生物科技有限公司,北京市 102200
- 折叠
摘要
Abstract
BACKGROUND: iRegene collagen sponge exhibits stable physical and chemical properties, and has passed the test by the State Food and Drug Administration of China. OBJECTIVE: To study the hemostatic effect and the biocompatibility of the iRegene collagen sponge on a liver wound by means of rat models. METHODS: Liver trauma bleeding models were made in 21 Sprague-Dawley rats. These model rats were randomized into three groups (n=7 per group): experimental group with implantation and external application of iRegene collagen sponge; positive control group with implantation of medical collagen sponge and external application of iRegene collagen sponge; blank control group with external application of medical gauze. The bleeding time and amount on the liver wounds were observed. Histological observation on the liver wound was performed at 7, 14, 28 days after intervention. RESULTS AND CONCLUSION: The bleeding time was shorter in the experimental group than the positive control group (P ≤ 0.05). Beyond that, there was no difference in the bleeding amount and time among the three groups. Histological findings on the liver wound showed that the iRegene collagen sponge in the experimental group was completely wrapped with fibrous connective tissues and began to degrade at 7 days after intervention, the Inflammatory cell infiltration mainly occurred in neutrophils, and new capillaries were observed in peripheral connective tissues; at 14 days after intervention, the fibrous connective tissues became remarkably thickened, the number of neurophils was reduced, and the number of macrophages was increased; at 28 days after intervention, the iRegene collagen sponge degraded completely, most of the liver tissues recovered, and there were macrophages, monocytes, fibroblasts and capillaries in the inflammatory connective tissues adjacent to a part of liver tissues. Similar findings were observed in the positive control group. In the blank control group, there were obvious connective tissues on the wound and red blood cells in the liver sinus, and occasionally liver tissue bleeding and vacuolar degeneration were visible; at 28 days after intervention, there were thickened connective tissues on the wound, red blood cells in the liver sinus and reversed hepatic stellate cells. To conclude, the iRegene collagen sponge possesses effective hemostatic effects on liver wounds and shows good histocompatibility.关键词
生物材料/材料相容性/胶原蛋白海绵/止血/肝脏创伤/组织相容性分类
医药卫生引用本文复制引用
张自强,张以河,祖耀先,安琪,徐荣荣..胶原蛋白海绵对肝脏创伤止血的效果[J].中国组织工程研究,2017,21(30):4873-4878,6.
