湖北医药学院学报2017，Vol.36Issue(4)：287-290,封2,5.DOI:10.13819/j.issn.1006-9674.2017.04.001
TET1基因敲除对宫颈癌细胞增殖迁移能力的影响
Effect of TET1 Gene Knockout on the Proliferation and Migration of Cervical Cancer Cells
摘要
Abstract
Objective To study the expression and role of TET1 in cervical cancer.Methods The proliferation gene of HeLa cells was tested by CRISPR/Cas9,and the proliferation status of cells was dynamically measured by real-time cell analyzer (RTCA).The effect of TET1 gene knockout on the migration ability of HeLa cells was detected by scratching test.Results The TET1 gene was successfully targeted by CRISPR/Cas9.TET1 knockout can increase the proliferation,invasion and migration of HeLa cells.Concltsion TET1 knockdown could increase the malignant behavior of cervical cancer HeLa cells.关键词
宫颈癌/TET1/CRISPR/Cas9Key words
Cervical cancer/TET1/CRISPR/Cas9引用本文复制引用
丁妍,于莉,周军阳,李东升,周青珍..TET1基因敲除对宫颈癌细胞增殖迁移能力的影响[J].湖北医药学院学报,2017,36(4):287-290,封2,5.基金项目
国家自然科学基金(81602297) （81602297）
湖北省科技厅面上项目(2016CFB411) （2016CFB411）
