氯喹通过诱导凋亡增强5-氟尿嘧啶的抗结肠癌效果OA
Chloroquine Enhances the Antitumor Effect of 5-fluorouracil on Colon Cancer through Inducing Apoptosis
目的:探讨结肠癌化疗药物5-氟尿嘧啶(5-fluorouracil,5-FU)与各个阶段的自噬抑制剂联合使用后,结肠癌细胞的增殖和凋亡情况,以寻找合适的治疗结肠癌的药物.方法:自噬三个阶段的抑制剂与5-FU联合使用处理结肠癌细胞系HT-29和SW480后,MTT方法检测细胞增殖情况,流式细胞术检测细胞凋亡情况,western blot检测细胞自噬和凋亡情况.结果:5-FU能诱导结肠癌细胞的自噬和凋亡.3-甲基腺嘌呤(3 methyladenine…查看全部>>
Objective To investigate the effects of 5-fluorouracil (5-FU) combined with autophagy inhibitors in various stages on proliferation and apoptosis of colon cancer cells thereby finding a suitable drug for colon cancer.Methodls HT29 and SW480 cells were treated with the combined use of autophagy inhibitors in three stages with 5-FU.The proliferation rate of cancer cells was analyzed by MTT assay.Apoptosis was quantified by flow-cytometry after the cells …查看全部>>
刘阅文;杜伯雨;郗雪艳;刘丹丹
湖北医药学院基础医学院,湖北十堰442000湖北医药学院基础医学院,湖北十堰442000湖北医药学院基础医学院,湖北十堰442000湖北医药学院基础医学院,湖北十堰442000
结肠癌自噬凋亡5-氟尿嘧啶氯喹
Colon cancerAutophagyApoptosis5-fluorouracilChloroquine
《湖北医药学院学报》 2017 (4)
291-295,299,6
2016年湖北省教育厅指导项目(B2016504)2016年湖北医药学院大学生创新创业训练计划项目(201613249003)
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