中国临床药理学杂志2017，Vol.33Issue(17)：1634-1636,1650,4.DOI:10.13699/j.cnki.1001-6821.2017.17.007
亚甲基四氢叶酸还原酶基因多态性与急性淋巴细胞白血病患者甲氨蝶呤化疗药物不良反应的相关性研究
Association of methylenetetrahydrofolate reductase polymorphism and adverse reaction of methotrexate chemotherapy in acute lymphocytic leukemia
摘要
Abstract
Objective To study the association of methylenetetrahydrofolate reductase (MTHFR) gene C677 T polymorphism and adverse reaction of high-dose methotrexate (HD-MTX) chemotherapy in acute lymphocytic leukemia(ALL).Methods Ninety-three blood samples of ALL patients were collected.Kit assay was used to study the DNA by blood samples.Polymerase chain reaction-microarray hybridization technology was used to detect the polymorphism of MTHFR C677T.Enzyme multiplied immunoassay (EMIT) technique was used to monitor dynamic se-rum concentration of patients.Clinical data of the ALL patients were collected and adverse effect were recorded to observe the incidence of adverse effect during the chemotherapy of HD-MTX.ANOVA and Logistic regression were used to analyze the association of polymorphism in MTHFR C677T gene and adverse reaction by HD-MTX.Results There was significant difference between MTHFR C677 T and mucositis.The risk of mucositis was higher in carriers of MTHFR 677CT + TT genotype as compared with patients with 677CC genotype,CT genotype was higher above grade 2.81 (OR =2.81,95％ CI:1.06-7.40,P ＜ 0.05) as compared with CC genotype,and TT genotype was higher above grade 5.53(OR =5.53,95％CI:1.58-19.27,P ＜0.05).The incidence of Ⅱ myelosuppression in TT genotype was higher above grade 3.75 (OR =3.75,95％ CI:1.13-12.40,P ＜ 0.05) as compared with CC genotype.There was significant difference about the dynamic serum concentration of 48h after HD-MTX chemotherapy among the MTHFR C677 T genotype (P ＜ 0.05).Conclusion The MTHFR C677 T polymorphism may have influence on the dynamic serum concentration of MTX.There was some relevance between MTHFR C677T polymorphism and adverse reactions (mucositis and Myelosuppression) after chemotherapy with HD-MTX.关键词
亚甲基四氢叶酸还原酶/基因多态性/大剂量甲氨蝶呤/药物不良反应Key words
methylenetetrahydrofolate reductase/genetic polymorphism/high-dose methotrexate/adverse reaction分类
医药卫生引用本文复制引用
李静,王捷,郁长治,赵军,袁圆,陈渤松,王建华..亚甲基四氢叶酸还原酶基因多态性与急性淋巴细胞白血病患者甲氨蝶呤化疗药物不良反应的相关性研究[J].中国临床药理学杂志,2017,33(17):1634-1636,1650,4.基金项目
新疆维吾尔自治区卫生厅青年科技人才专项科研基金资助项目(2014Y23) （2014Y23）
