中国临床药理学杂志2017，Vol.33Issue(17)：1675-1678,4.DOI:10.13699/j.cnki.1001-6821.2017.17.019
内毒素亲和吸附剂对创伤失血性休克大鼠肠损害及炎症因子水平的影响
Effect of endotoxin affinity adsorbent on intestinal damage and inflammatory factors in rats with traumatic hemorrhagic shock
摘要
Abstract
Objective To investigate the effect of endotoxin affinity adsorbent (EAA) on intestinal injury and inflammatory factors in rats with traumatic hemorrhagic shock.Methods A total of 50 SD rats were randomly divided into five groups:normal group,model group,experimental low-dose (experimental-L group),middle-dose (experimental-M group) and high-dose (experimental-H group) groups,10 rats in each group.Rats used for traumatic hemorrhagic shock model establishing were treated by making trauma and bloodletting.Before 30 min with model establishing,rats in the experimental-L,-M and-H groups were given (FAA 5,10,15 mL +15,10,5 mL 0.9％ NaCl)respectively.The morphological changes of intestinal villi and intestinal mucosa damage in rats of those different groups were observed by HE staining.Resuscitation after shock for 4,8 and 16 h,the changes of inflammatory factor levels,such as endotoxin,nitric oxide(NO) and tumor necrosis factor α (TNF-α),in different groups were observed.Results Compared with blank group(0.03 ±0.01),the intestinal mucosal injury index in model group was 5.41 ±2.56 with significandy(P ＜ 0.05).Resuscitation after shock for 4 h,compared with model group,the intestinal mucosal injury index in experimental-H,experimental-M,experimental-L groups were 3.45 ±0.75,2.53 ±0.68,2.02 ±0.53 with significantly(all P ＜0.05).Compared with blank group,the endotoxin,NO and TNF-α in model group were (456.52 ± 21.56) pg · mL-1,(108.41 ± 9.56) ng · mL-1,(77.58 ± 10.65)ng· mL-1 with significantly (all P ＜ 0.05).Resuscitation after shock for 4 h,the endotoxin,NO and TNF-α in experimental-H,experimental-M,experimental-L groups were (280.41 ± 15.42),(254.63 ± 17.56),(221.53 ± 13.53)pg · mL-1;(90.43 ± 8.42),(63.54 ± 7.53),(49.52 ± 8.41) ng · mL-1;(77.58 ± 10.65),(62.58 ±5.12),(42.51 ±4.53)ng · mL-1,respectively.Compared with model group,the endotoxin,NO in three experimental groups had significantly (all P ＜ 0.05) while TNF-α in experimental-M,experimental-L groups had significantly(all P ＜0.05).Conclusion low-dose EAA could effectively relieve the stress response caused by hemorrhagic shock and reduce the level of inflammatory factors,thus it could protect the intestinal function of rats.关键词
内毒素亲和吸附剂/失血性休克/肠损伤/炎症因子Key words
endotoxin affinity adsorbent/hemorrhagic shock/intestinal injury/inflammatory factor分类
医药卫生引用本文复制引用
刘冰冰,刘海,张建新,彭晓静,郭平选..内毒素亲和吸附剂对创伤失血性休克大鼠肠损害及炎症因子水平的影响[J].中国临床药理学杂志,2017,33(17):1675-1678,4.基金项目
河北省医学科学研究重点课题计划基金资助项目(ZD20140064) （ZD20140064）
