中国临床药理学杂志2017，Vol.33Issue(17)：1679-1682,1686,5.DOI:10.13699/j.cnki.1001-6821.2017.17.020
当归挥发油通过激活ERK信号通路以减轻缺血再灌注神经细胞凋亡的作用
Function of essential oils from Angelica sinensis reduce apoptosis on the neural cells of damaged by ischemia-reperfusion like injury through up-regulation of ERK pathway
摘要
Abstract
Objective To study the effect of essential oil from Angelica sinensis(EOAS) on neuronal apoptosis induced by ischemia-reperfusion injury.Methods The high differentiated PC12 cells were divided into blank group,model group,control group (10 μmol · L-1 edaravone)and large-,medium-and small-dose expreimental groups(EOAS 25.00,12.50,6.25 μg · mL-1).Except blank group,the remaining groups were treated with sugar-free medium containing 10 mmol · L-1sodium dithionite (Na2S204) for 1 h,reoxygenation for 48 h.The cell proliferation was measured by methyl thiazolyl tetrazolium(MTT) assay.The activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH),contents of malondialdehyde (MDA) were measured by kit.The apotosis rate and the morphological apoptotic characteristics of cells were observed by flow cytometry and acridine orange/propidium iodide (AO/PI) double staining respectively.The relative activity of caspase-3 were examined with colorimetric assay.The expression of p-ERK1/2 proteins was measured by Western-blot.Results Compared with the blank group,the cell viability in the model group was (67.4 ±0.10)％ with significantly(P ＜0.01).Compared with the model group,the cell viability in the large-dose experimental group and control group were (86.2 ± 0.10)％,(94.5 ± 0.05)％ with significantly (P＜0.05,P ＜ 0.01).Compared with the blank group,the LDH and MDA content in the model group were (912.53±16.71)U · L-1and(9.05 ±0.25)μmol · L-1while SOD level was (12.53 ±0.29)U · mL-1with significantly(all P ＜0.01).Compared with the model group,the LDH and SOD level in the large-dose experimental group were (565.61 ± 11.72) U · L-1 and (12.53 ± 0.29) U · mL-1 with significantly (P ＜ 0.05,P ＜ 0.01) while MDA content in the large-,medium-dose experimental groups were (3.32 ± 0.68),(5.79 ± 0.68) μmol · L-1 with significantly(all P ＜0.01).The absorbance(A) in model group and large-,medium-and small-dose expreimental groups were 0.75 ± 0.06 and 0.10 ± 0.02,0.16 ± 0.03,0.49 ± 0.04.Compared with the model group,the difference had significantly(P ＜0.05,P ＜0.01).Compared with the blank group,the apoptosis rate in the model group was (31.17 ± 2.44)％ with significantly(P ＜ 0.01).Compared with the model group,the apoptosis rate in the large-,medium-dose experimental groups were (4.57 ± 0.32) ％,(5.93 ± 0.81) ％ with significantly (all P ＜ 0.01).Compared with the model group,p-ERK1/2 proteins of large-dose experimental group were up-regulated significantly (P ＜0.01).Conclusion The EOAS could inhibit the apoptosis of neurons after ischemia-reperfusion by activating ERK signaling pathway.关键词
当归挥发油/PC12细胞/拟缺血再灌注/细胞凋亡/ERK信号通路Key words
essential oils from Angelica sinensis/PC12 cell/ischemia-reperfusion like injury/apoptosis/ERK pathway分类
医药卫生引用本文复制引用
朱丽娟,罗建云,张安平,石皓,宋润泽,臧凯宏..当归挥发油通过激活ERK信号通路以减轻缺血再灌注神经细胞凋亡的作用[J].中国临床药理学杂志,2017,33(17):1679-1682,1686,5.基金项目
甘肃省青年科技基金资助项目(1308RJYA035) （1308RJYA035）
甘肃省高等学校科研基金资助项目(2014B-056) （2014B-056）
甘肃中医药大学中青年科研基金资助项目(ZQ2014-1) （ZQ2014-1）
