中国临床药理学杂志2017，Vol.33Issue(17)：1683-1686,4.DOI:10.13699/j.cnki.1001-6821.2017.17.021
白藜芦醇诱导细胞外调节蛋白激酶信号通路以缓解帕金森病神经元损伤的研究
Research on extracellular regulatory protein kinase signaling pathway induced by resveratrol in alleviating neuronal damage in Parkinson's disease
摘要
Abstract
Objective To investigate the effect of resveratrol by regulating the extracellular regulatory protein kinase (ERK) signaling pathway to alleviate neuronal damage in Parkinson's disease (PD) and its protective mechanism.Methods Forty male Wistar rats were induced with 6-hydroxydopamine to construct Parkinson's model,and they were randomly divided into model group and test group with 20 cases per group.Another 20 male Wistar rats were used as control group.Test group was given 20 mg· kg-1 · d-1 resveratrol,model and control groups were given 20 mg · kg-1 · d-10.9％ NaCl.Three groups were treated for 14 d with gavage.The changes of rotation behavior were observed between three groups.The levels of tyrosine hydroxylase were measured by immunohistochemistry.The levels of neuron apoptosis were measured by TUNEL method.The expression levels of ERK,nuclear factor-κB (NF-κB) and cysteinyl aspartate specific proteinase 3 (Caspase 3)were measured by Western blot.Results Mter treatment,the main indexes in test,model and control groups were compared:the average number of revolutions in 30 min wee (104.81 ± 6.64),(187.56 ± 7.32),(1.37 ± 0.45) cycles;tyrosine hydroxylase were (0.83 ± 0.04),(0.71 ± 0.06),(1.00 ± 0);neuron apoptosis were (18.23 ± 1.47),(32.84 ± 1.86),(1 1.58 ± 1.24) number;ERK protein were (1.57 ± 0.07),(1.14 ± 0.05),(1.32 ± 0.07);NF-κB protein were (0.86 ± 0.04),(1.08 ± 0.06),(0.79 ± 0.03);Caspase 3 protein were (0.68 ±0.03),(0.87 ±0.04),(0.59 ±0.02),the differences were statistically significant between test group and control,model groups (P ＜ 0.05).Conclusion Resveratrol can relieve neuronal damage in Parkinson's disease rats by regulating ERK signaling pathway.关键词
白藜芦醇/帕金森病/神经保护/细胞外调节蛋白激酶Key words
resveratrol/Parkinson's disease/neuroprotection/extracellular regulated protein kinase分类
医药卫生引用本文复制引用
郭景丽,郭英丽..白藜芦醇诱导细胞外调节蛋白激酶信号通路以缓解帕金森病神经元损伤的研究[J].中国临床药理学杂志,2017,33(17):1683-1686,4.基金项目
内蒙古自治区教育厅自然科学基金资助项目(NJZY16573) （NJZY16573）
