中国岩溶2017，Vol.36Issue(4)：572-579,8.DOI:10.11932/karst20170420
湖北清江和尚洞风化岩壁放线菌分离及基于16S rRNA的种类分析
Isolation and 16S rRNA gene sequence analysis of Actinomycetes from weathered rocks in Heshang Cave, central China
摘要
Abstract
The specific goal of this work was to investigate the actinobacterial diversity in the weathered rocks by culture-dependent approach in Heshang Cave,Hubei,China.Three weathered rocks samples (W1,W2 and W3) were collected from aphotic zone and twilight zone in Heshang Cave with sterile centrifuge tubes in August,2015.Five modified media and one self-designed medium of extracting of weathered rocks agar medium were used for isolation of actinomycetes in the weathered rocks of Heshang Cave.The isolates were identified by 16S rRNA gene sequencing using special primer for actinomycetes.Result shows that in total of 72 strains obtained by pure culture from 6 different media.The phylogenetic analysis showed that 72 actinomycetes sequences were classified into four genera in three orders of Actinobacteria with Streptomyces being dominative.The number of isolated strains in genera Streptomyces,Rhodococcus,Tsukamurella and Microbacterium was 69,1,1 and 1,respectively.Samples of W1 and W2 in aphotic zone were only isolated 9 and 10 strains respectively,while sample of W3 in twilight zone were obtained 53 strains.The results offer valuable information and isolates for the subsequent study on the interaction between carbonate and microbes.关键词
和尚洞/风化岩壁/放线菌/纯培养/16S rRNAKey words
Heshang Cave/weathered rocks/actinomycetes/pure culture/16S rRNA分类
生物科学引用本文复制引用
满百膺,程晓钰,王红梅,云媛,钟宇洪..湖北清江和尚洞风化岩壁放线菌分离及基于16S rRNA的种类分析[J].中国岩溶,2017,36(4):572-579,8.基金项目
国家自然科学基金重点基金(41130207) （41130207）
