中国药业2017，Vol.26Issue(21)：7-9,3.DOI:10.3969/j.issn.1006-4931.2017.21.003
雷帕霉素预处理对大鼠肢体缺血-再灌注肺损伤的影响
Effect of Rapamycin Pretreatment on Lung Injury Induced by Limb Ischemia-Reperfusion in Rats
摘要
Abstract
Objective To investigate the effect of rapamycin pretreatment on lung injury induced by limb ischemia-reperfusion in rats, to provide theoretical basis for clinical application and its prevention and treatment of lung injury induced by limb ischemia-reperfu-sion. Methods Totally 60 healthy male SD rats were divided into control group ( group C ) , limb ischemia reperfusion group ( group I/R ) , rapamycin pretreatment group (group R) by random number table method, 20 cases in each group. 12 h before ischemia, all were fasted, free drinking water and fixed on the operating table after anesthesia. In the right groin skin incision, the exposed femoral artery, femoral vein and femoral nerve. Only femoral artery, femoral vein and femoral nerve separated in group C, the femoral artery was clamped at the proximal in-guinal ligament with a noninvasive vascular clamp in group I/R and group R, in order to force the hind limb of the rats to undergo ischemia for 2 h, and the vascular clamp was removed to make the reperfusion for 3 h. In group R, rapamycin was injected into the lower limb before ischemia. TTFM was used to monitor blood flow, no blood flow was monitored for ischemia, and blood flow was monitored as an indicator of reperfusion. After reperfusion for 3 h, the rats were sacrificed, and 2 mL blood was taken from the heart, the blood serum was taken by cen-trifuging. The concentration of MDA was determined by thiobarbituric acid method, and the levels of IL-1β, IL-6 and TNF-α were detected by ELISA. The lung tissue was taken, and the pathological results of lung tissue were observed by calculating the ratio of dry-wet and im-munohistochemistry. Results Compared with group C, theconcentration of W/ D in lung, serum MDA, IL-1β, IL-6 and TNF-α in group I/R increased significantly( P < 0. 01) . Compared with I/R group, the concentration of W/ D in lung, serum MDA, IL-1β, IL-6 and TNF-α in group R decreased significantly( P < 0. 05). Under light microscope, the structure of lung tissue in group C was intact, and the alveolar septa were uniform. A few inflammatory cells were infiltrated, and the alveolar cavity was clearly visible. In group I/R, the proliferation of alveolar epithelial cells, the obvious interstitial proliferation of lung and the thickening of alveolar wall wereobserved, a large number of inflammatory cell infiltration and capillary expansion and congestion wereobserved, and a large number of neutrophils were found in the alveolar wall and alveolar stroma. The pathological changes of lung tissue in group R were significantly less than those in group I/R, the infiltration of inflam-matory cells was significantly reduced, only a small amount of red blood cells were exuded. Conclusion Rapamycin pretreatment can reduce lung injury induced by limb ischemia-reperfusion, and its mechanism may be related to antioxidant stress and anti-inflammatory response.关键词
雷帕霉素/肢体缺血-再灌注/肺损伤Key words
rapamycin/limb ischemia-reperfusion/lung injury分类
医药卫生引用本文复制引用
袁志军,唐斌,罗振中,黄丹..雷帕霉素预处理对大鼠肢体缺血-再灌注肺损伤的影响[J].中国药业,2017,26(21):7-9,3.基金项目
江西省卫生计生委科技计划项目[ 20165283 ]. （）
