浙江医学2017，Vol.39Issue(18)：1513-1517,5.DOI:10.12056/j.issn.1006-2785.2017.39.18.2016-1373
FTY720对蛛网膜下腔出血大鼠神经功能的作用及机制
Effect of FTY720 on neurological function in rats with subarachnoid hemorrhage and related mechanism
摘要
Abstract
Objective To investigate the effect of FTY720 on neurological function in rats with subarachnoid hemorrhage (SAH) and the related mechanism.Methods A total of 96 rats were randomly assigned to normal group,sham-operation group,model group and treatment group with 24 rats in each group.The rat SAH model was established via autologous blood injection into cisterna magna.Rats in treatment group were given intraperitoneally with 1mg/kg FTY720.Rats in other three groups were given intraperitoneal injection of 1ml normal saline.After 24 hours,12 rats in each group were sacrificed by decapitation and their hippocampus tissue samples were collected.Western blot was used to determine the expression of nuclear factor-kappa B (NF-κB).Immunohistochemistry was utilized to investigate the microglial OX-42 expression.Other 12 rats in each group experienced neurological function assessment.Results The relative expressions of NF-κB and microglial OX-42 in the hippocampus tissues were significantly higher in model group than those in normal group and sham-operation group (all P＜0.05),also higher than those in treatment group (all P＜0.05).Forelimb-placement test,balance test and modified neurological severity scores at d 7,14,21 and 28 postoperatively in treatment group were all significantly lower than those in model group (all P＜0.05).Conclusion FTY720 can markedly improve the neurological function of rats with SAH,which may be related to the inhibition of inflammation in central nervous system.关键词
FTY720/大鼠/蛛网膜下腔出血/神经功能/炎症反应/脑保护作用Key words
FTY720/Rat/Subarachnoid hemorrhage/Neurological function/Inflammation/Cerebral protective effect引用本文复制引用
董晓巧,朱强,车志豪,孙承龙,刘群杰,王鼎,俞文华,杜权,王昊,杨定博,沈永锋,江力,胡强,杜垣锋..FTY720对蛛网膜下腔出血大鼠神经功能的作用及机制[J].浙江医学,2017,39(18):1513-1517,5.基金项目
浙江省实验动物科技计划项目(2015C37082) （2015C37082）
