浙江医学2017，Vol.39Issue(18)：1518-1523,后插2,7.DOI:10.12056/j.issn.1006-2785.2017.39.18.2016-2010
miR-181家族在胶质母细胞瘤中的表达及其临床意义
Expression of miR-181 family in glioblastoma and its clinical significance
摘要
Abstract
Objective To investigate the expression of miR-181 family in multiform glioblastoma(GBM)and its correlation with clinicopathological characteristics of the tumor.Methods The expression of miR-181a,miR-181b,miR-181c and miR-181d was detected by loop RT-qPCR in tumor tissue specimens of 40 GBM patients.The relationship between miR-181 expression and clinicopathological characteristics was analyzed.The lentiviral vector carrying miR-181a was transfected into U251 cells,the proliferation clone-forming ability and apoptosis of miR-181a-transfected U251 cells were determined.Results The up-regulation of miR-181a expression was correlated with the survival of patients and peritumoral brain edema (P＜0.05).Expression of miR-181a was significantly higher in patients with O6-methylguanine-DNA methyltransferase(MGMT) positive than those with MGMT negative (P＜0.05).Cell studies indicated that the over-expression of miR-181a significantly inhibited the proliferation of U251 cells(P＜0.01),reduced clone-forming ability (P＜0.05),and increased apoptosis (P＜0.05).Conclusion The expression of MiR-181a may be associated with the prognosis of GBM,and down-expression of miR-181a may be involved in the development of GBM.关键词
miR-181/胶质母细胞瘤/MGMTKey words
miR-181/Glioblastoma/MGMT引用本文复制引用
王成德,李群,卢江龙,蔡霖,高志超,苏志鹏..miR-181家族在胶质母细胞瘤中的表达及其临床意义[J].浙江医学,2017,39(18):1518-1523,后插2,7.基金项目
浙江省自然科学基金项目(LY16H160053) （LY16H160053）
浙江省医药卫生科技计划项目(2012RCA042) （2012RCA042）
温州市科技计划项目(Y20120008) （Y20120008）
