浙江医学2017，Vol.39Issue(18)：1524-1526,3.DOI:10.12056/j.issn.1006-2785.2017.39.19.2016-1656
脑胶质瘤组织时钟基因启动子区甲基化状态及意义
Methylation status of clock gene promoter region in glioma patients
摘要
Abstract
Objective To investigate the methylation status of PER1,PER2,CRY1,CRY2,CLOCK,BMAL1 and NPAS2 gene promoter regions in glioma tissues and its clinical significance.Methods The frequencies of methylation of PER1,PER2,CRY1,CRY2,CLOCK,BMAL1 and NPAS2 gene promoters were determined in glioma tissues and in surrounding non-glioma tissues (control group) were detected by methylation-specific PCR method.Results PER1 and CLOCK genes were devoid of methylation in promoter region.Differences in the promoter methylation frequencies of CRY1,CRY2 and BMAL1 genes between glioma and control group were not significant (P＞0.05).Promoter methylation frequencies of NPAS2 and PER2 genes were significantly were elevated in glioma tissue compared to control group(P＜0.05),and moreover,promoter methylation frequency of NPAS2 was enhanced with increasing extent of malignancy(P＜0.05).Conclusion DNA-methylation modification in the promoter of NPAS2 and PER2 genes may be an important mechanism underlying occurrence and development of glioma.关键词
脑胶质瘤/启动子区/甲基化/时钟基因Key words
Glioma/Promoter/Methylation/Clock gene引用本文复制引用
杜权,俞文华,董晓巧,朱强,车志豪,王昊,杨定博,沈永锋,江力..脑胶质瘤组织时钟基因启动子区甲基化状态及意义[J].浙江医学,2017,39(18):1524-1526,3.基金项目
浙江省医药卫生科技计划项目(2014KYA168) （2014KYA168）
