浙江医学2017，Vol.39Issue(18)：1527-1531,1535,6.DOI:10.12056/j.issn.1006-2785.2017.39.18.2017-128
二氢睾酮诱导小鼠雄激素性脱毛的机制研究
Mechanism of androgenetic alopecia in mouse model
摘要
Abstract
Objective To explore the mechanism of androgenetic alopecia (AGA) in mouse model.Methods AGA model was induced by subcutaneous injection of dehydrotestosterone (DHT) with concentration gradient method in 112 female mice.Hair depilation method was used to synchronize the initial stage of hair growth cycle of injected mice.The morphology and number of follicle were observed by H.E staining,and the mRNA expressions of signaling molecules in hair follicles were detected by RT-PCR.Results The follicular miniaturization and hair thinning were observed in mice treated with different concentration of DHT throughout the hair cycle.RT-PCR results showed the expression of androgen receptor (AR) was increased in whole hair cycle,but decreased at d40 and d48.The mRNA expression of stem cell marker Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) was decreased during telogen and anagen Vl phases,while the expression of CD34 had no significant changes.The expressions of FGFR,IGF-BP5,Ptch1 and Wnt were reduced compared with control group.Coinciding with decreased AR expression at d40 and d48,the levels of PDGFRα were significantly declined (P＜0.05).The expression of basement membrane components laminin α 3 was up-regulated,and laminin α 5 was down-regulated in DHT-induced AGA mouse.Conclusion DHT can stably induce androgenetic alopecia in mice.DHT can inhibit the expression of hair growth factor FGFR,IGF-BP5,Ptch1 and Wnt signaling by activating the AR in DP cells and interacting with epithelial cells in the hair follicle.关键词
二氢睾酮/雄激素受体/脱毛/模型/信号通路Key words
Dehydrotestosterone/Androgen receptor/Alopecia/Modelm/Signal pathway引用本文复制引用
林蕾,杨梦洁,吴骁,王少敏,倪曙民,叶孟,高晶..二氢睾酮诱导小鼠雄激素性脱毛的机制研究[J].浙江医学,2017,39(18):1527-1531,1535,6.基金项目
国家自然科学基金(81101206) （81101206）
宁波市自然科学基金(2013A610253) （2013A610253）
