浙江医学2017，Vol.39Issue(18)：1532-1535,4.DOI:10.12056/j.issn.1006-2785.2017.39.18.2016-1085
2型糖尿病大鼠膀胱逼尿肌中膜结合型前列腺素E2合酶1的表达及意义
Expression of mPGES-1 in bladder of streptozotocin-induced diabetic rats
摘要
Abstract
Objective To investigate the expression of membrane-associated prostaglandin synthase 1 (mPGES-1) in urinary bladder of streptozotocin (STZ)-induced diabetic rats.Methods Thirty six female SD rats were randomly divided into model and control groups with 18 in each.Diabetic cystopathy (DCP) model was induced by intraperitonieal injection of streptozotocin (STZ).After 12 weeks,the animals were sacrificed and the bladder samples were harvested.The histological changes were observed by light microscopic and electron microscopic examination,and the expression of mPGES-1 in bladder tissue was detected by immunohistochemistry and the contents of PGE2 in 24h urine were measured by ELISA method.Results Compared with the control group,diabetic rats showed disorder of detrusor smooth muscle structure and wider muscle gap under light microscope.Electron microscopy showed that intercellular space of detrusor was wider and detrusor cells was irregular in shape,the swelling and rupture of mitochondria,and vacuolization were found in cytoplasm.Compared to control group,the contents of PGE2 in the urine and the expression of mPGES-1 in bladder detrusor tissue of DCP group were significantly decreased.Conclusion The down-regulated expression of mPGES-1 may play an important role in the occurrence and development of diabetic cystopathy in rats.关键词
2型糖尿病/膀胱/膜结合型前列腺素E2合酶1/前列腺素E2Key words
Type 2 diabets/Bladder/mPGES-1/Prostaglandin E2引用本文复制引用
王彦彬,李瑞鹏,诸靖宇,徐侃..2型糖尿病大鼠膀胱逼尿肌中膜结合型前列腺素E2合酶1的表达及意义[J].浙江医学,2017,39(18):1532-1535,4.基金项目
浙江省医学会临床科研基金项目(2013ZYC-A41) （2013ZYC-A41）
杭州市医学重点学科基金资助项目(2014-13-65、2017-51-84) （2014-13-65、2017-51-84）
