浙江医学2017，Vol.39Issue(18)：1544-1547,4.DOI:10.12056/j.issn.1006-2785.2017.39.18.2017-41
结直肠癌中miR-21表达与临床病理因素及预后的关系
Expression of miR-21 in colorectal cancer and its clinicopathologic significance
摘要
Abstract
Objective To investigate the expression of miR-21 in colorectal cancer and its association with clinicopathological characteristics and the prognosis of patients.Methods The expression of miR-21 in 59 specimens of colorectal cancer and matched non-tumor adjacent tissue specimens was examined with stem-loop real-time RT-PCR.The relationship between the expression of miR-21 and clinicopathologic features of colorectal carcinoma was analyzed.Kaplan-Meier survival curves,log-rank test and multivariate Cox regression were used to analyze the factors related to prognosis of colorectal cancer.Results The expression level of miR-21 was significantly higher in colorectal cancer tissue than that in the matched normal colorectal mucosa (2.698 ± 1.973 vs.0.653 ± 0.213,P＜0.05).The expression of miR-21 was positively correlated with tumor size,lymph node metastasis and clinical stage (P＜0.05),but not with sex,distant metastasis,cell differentiation,depth of tumor infiltration and location (P ＞0.05).Kaplan-Meier univariate analysis showed that distant metastasis,cell differentiation,depth of tumor infiltration and miR-21 was the prognostic factor for colorectal cancer (P＜0.05).The Cox regression analysis showed that distant metastasis,cell differentiation and miR-21 was an independent prognostic risk factor of colorectal cancer(P＜0.05),RR value and 95％CIwas 5.053 (1.395-5.787),3.998 (1.395-18.305) and 3.837 (1.266-11.631).Conclusion The results indicate that miR-21 serves as a molecular marker for disease progression and prognosis of colorectal cancer.关键词
结直肠癌/miR-21/预后/Cox比例风险回归模型Key words
Colorectal cancer/miR-21/Prognosis/Cox model引用本文复制引用
任约翰,叶星照,厉金雷,陈积贤..结直肠癌中miR-21表达与临床病理因素及预后的关系[J].浙江医学,2017,39(18):1544-1547,4.基金项目
浙江省自然基金项目(Y2100917) （Y2100917）
