肿瘤防治研究2017，Vol.44Issue(9)：607-611,5.DOI:10.3971/j.issn.1000-8578.2017.17.0237
基于液相色谱-质谱联用技术的男性肺腺癌患者代谢组学研究
Metabonomics Investigation of Lung Adenocarcinoma in Male Patients Based on Liquid Chromatography-mass Spectrometry
摘要
Abstract
Objective To investigate the plasma metabolic profiles of lung adenocarcinoma in male patients by rapid resolution liquid chromatography coupled with time-of-flight tandem mass spectrometry(RRLC-QTOF-MS)-based metabolomics method,to provide the basis for early diagnosis.Methods Metabolomics analysis was carried out for serum samples between 20 healthy controls and 17 lung adenocarcinoma patients.Principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were carried out for pattern recognition and characteristic metabolites identification.Metabolic characteristics of patients with lung adenocarcinoma were investigated according to the differences of metabolic groups.Results Multivariate statistical analysis yielded a significant distinction in metabolic profiles between healthy and lung adenocarcinoma groups.The t-test results indicated a total of 16 biomarkers with statistically significant differences.Conclusion Based on the analysis of metabolic pathways,lysophosphatidylcholine,acylcarnitine,fatty acid and other substances in lung adenocarcinoma patients are disordered.These different metabolites could be helpful for diagnosing lung adenocarcinoma.关键词
代谢组学/肺腺癌/液相色谱-质谱/诊断/生物标志物Key words
Metabolomics/Lung adenocarcinoma/LC-MS spectrometry/Diagnosis/Biomarkers分类
医药卫生引用本文复制引用
孙守坤,丛立新,李明晶,陈卓,郭环宇..基于液相色谱-质谱联用技术的男性肺腺癌患者代谢组学研究[J].肿瘤防治研究,2017,44(9):607-611,5.基金项目
吉林省科技计划项目(20130102099JC) （20130102099JC）
吉林省卫生科研计划项目(20132102) （20132102）
