中药材2017，Vol.40Issue(6)：1300-1304,5.DOI:10.13863/j.issn1001-4454.2017.06.013
安徽霍山产3种石斛多糖组成的比较研究
Comparison of Polysaccharide Composition of Three Kinds of Dendrobium Plants Originated in Huoshan, Anhui
摘要
Abstract
Objective:Huoshan in Anhui was the extreme north of geographic distribution for Dendrobium plants,produced three kinds Dendrobium plants,Dendrobium huoshanense,Dendrobium moniliforme and Dendrobium officinale,their polysaccharide extract had significant pharmacological activity,this study aimed to explore the similarities and differences on the polysaccharide composition of three kinds of Dendrobium plants in the same habitat.Methods:Wintering stem was selected to tested material,differences of content of three kinds of Dendrobium polysaccharides were analyzed by phenol-sulfuric acid method,differences of molecular weight distribution of three kinds of Dendrobium polysaccharides were analyzed by high performance size exclusion chromatography(HPSEC).Differences of monosaccharide composition of three kinds of Dendrobium polysaccharides were analyzed by column derivatization HPLC.Results:The polysaccharides content accounted for(18.65 ± 1.50)％,(11.32 + 1.30)％ and(20.78 + 1.60) ％ of dry weight,respectively in Dendrobium huoshanense,Dendrobium moniliforme and Dendrobium officinale,and their molecular weight distribution range overlap each other,and the proportion of highly active polysaccharide whose molecular weight between 50 ～200 kDa in three kinds of Dendrobium polysaccharides was(16.38 + 1.40) ％,(21.61 ± 1.30) ％ and(14.19 ± 1.50) ％,respectively.Total seven monosaccharides including glucose (Glc),mannose (Man),galactose (Gal),xylose (Xyl),glucuronic acid (GlcUA),galacturonic acid (GalUA) and Arabian sugar (Ara),were detected in polysaccharide hydrolysate of three kinds Dendrobium,and mannose and glucose were all the main monosaccharide components,but the relative peak area ratio of mannose and glucose of Dendrobium officinale was the largest.Conclusion:The content and molecular weight distribution and monosaccharide composition of three kinds of Dendrobium polysaccharide have both similarities and obviously differences in the same habitat.The experiment results provide important reference for identifying and exploring the quality differences,especially the group activity relationship,of three kinds of Dendrobium species.关键词
霍山产石斛/多糖/分子量分布/单糖组成Key words
Dendrobium originated in Huoshan/Polysaccharides/Molecular weight distribution/Monosaccharide composition分类
医药卫生引用本文复制引用
邓辉,韩邦兴,陈乃东,谷仿丽,戴军,陈乃富..安徽霍山产3种石斛多糖组成的比较研究[J].中药材,2017,40(6):1300-1304,5.基金项目
国家自然科学基金(81274021,81573536) （81274021,81573536）
安徽省教育厅自然科学基金(KJ2015ZD43,KJ2014A279) （KJ2015ZD43,KJ2014A279）
2017安徽高校优秀青年人才支持计划项目(gxyq2017057) （gxyq2017057）
皖西学院自然科学基金(WXZR201616) （WXZR201616）
