中药材2017，Vol.40Issue(6)：1339-1344,6.DOI:10.13863/j.issn1001-4454.2017.06.022
基于UPLC-Q-TOF-MS法分析江西虫草化学成分
Study on Chemical Compositions in Cordyceps jiangxiensis by UPLC-Q-TOF-MS
摘要
Abstract
Objective:To analyze and identify the main chemical compositions of Cordyceps jiangxiensis by UPLC-Q-TOF-MS.Methods:Using Agilent SB-C18 (50 mm× 4.6 mm,1.8 μm) column with gradient elution of 0.1％ formic acid(A)-acetonitrile (B),the flow rate was 0.25 mL/min.The column temperature was set at 30 ℃.MS analysis was based on information associated mode (IDA),and positive and negative ions were collected respectively.Results:Combining feature of PeakView such as Formula Finder,matching the Mass spectrometry data of each chromatographic peak in the database,and the cleavage law of each peak's level two fragment,which identified 29 compounds in Cordyceps jiangxiensis,twelve compounds were first reported from Cordyceps substance.The main chemical constituents include alkaloids,sterols,glycosides,nucleosides,amino acids,etc.Conclusion:The method is accurate,reliable,and effficient,and is suitable for rapid identification of ingredients in Cordyceps jiangxiensis,which provide a reference for the development and utilization of Cordyceps jiangxiensis and clarify its efficacy and material basis.关键词
江西虫草/UPLC-Q-TOF-MS/定性分析Key words
Cordyceps jiangxiensis Z.Q.Liang,A.Y.Liu & Y.Ch.Jiang/UPLC-Q-TOF-MS/Qualitative analysis分类
医药卫生引用本文复制引用
秦伟瀚,刘飞,刘翔,张小梅,阳勇,花雷..基于UPLC-Q-TOF-MS法分析江西虫草化学成分[J].中药材,2017,40(6):1339-1344,6.基金项目
国家科技重大专项课题(2012ZX09103201-050) （2012ZX09103201-050）
重庆市科学技术委员会资助项目(2015cstc-jbky-019013,2015cstc-jbky-01917) （2015cstc-jbky-019013,2015cstc-jbky-01917）
重庆市卫生局中医药科技项目(ZY20132071) （ZY20132071）
