中药材2017，Vol.40Issue(6)：1410-1414,5.DOI:10.13863/j.issn1001-4454.2017.06.039
松脂醇二葡萄糖苷对UVB诱导HDF细胞损伤的保护作用及机制研究
Study on Protctive Effects and Mechanism of PDG on UVB Induced HDF Cells Damage
摘要
Abstract
Objective:To investigate the protective effect and the mechanism of PDG on UVB induced HDF cells injury.Methods:The rate of cell proliferation was determined by MTT assay,the collagen protein in cells,matrix metalloproteinases-1 (MMP-1),ERβ,p-P38 protein expression level were measured by Western blot,and the expression of MMP-1 mRNA was detected by real-time fluorescent quantitative PCR method.Results:Compared with the control group,the cell proliferation rate and ERβ,COL Ⅰ protein expression in model group decreased obviously,MMP-1 and p-P38 protein expression and MMP-1 mRNA expression increased significantly (P ＜0.01).Compared with the model group,cell proliferation rate and collagen of PDG group increased significantly.At the same time,PDG inhibited the expression of MMP-1 protein and MMP-1 mRNA,and up-regulated the expression of ERβ protein and decreased p-P38 protein expression significantly(P ＜ 0.01).Compared with the PDG group,ICI182780 was significantly suppressed ERβ protein expression and up-regulated MMP-1 mRNA expression,and SB203580 significantly inhibited the expression of MMP-1 and p-P38 protein,and decreased mRNA expression of MMP-1 (P ＜ 0.01).Conclusion:The results indicated that PDG can significantly promote the proliferation rate of HDF cells,and mechanism may through the ER-P38MAPK signaling pathway inhibiting expression of MMP-1 protein and MMP-1 mRNA,to promote the synthesis of collagen to achieve the purpose of prevention and treatment of light aging.关键词
松脂醇二葡萄糖苷/雌激素受体/MAPK信号通路/皮肤光老化Key words
Pinoresinol diglucoside (PDG)/Estrogen receptor/MAPK signaling pathway/Photoaging分类
医药卫生引用本文复制引用
刘俊岑,董蕊,朴成玉,张智博,牛春影,耿放,张宁..松脂醇二葡萄糖苷对UVB诱导HDF细胞损伤的保护作用及机制研究[J].中药材,2017,40(6):1410-1414,5.基金项目
国家自然科学基金面上项目(81274035) （81274035）
黑龙江省博士后科研启动基金(LBH-Q13162) （LBH-Q13162）
黑龙江中医药大学优秀创新人才支持计划(2012) （2012）
